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Mouse uPAR Antibody

Catalog #: MAB531
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB531-SP
MAB531-100
MAB531-500
Detection of uPAR in Mouse Blood Monocytes by Flow Cytometry.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse uPAR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse uPAR in Western blots. In Western blots, no cross-reactivity with recombinant human uPAR is observed.
Source
Monoclonal Rat IgG2A Clone # 109801
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse uPAR isoform 1
Leu24-Thr297
Accession # P35456
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse uPAR Fc Chimera (Catalog # 531-PA)
under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
See below
Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.5-2.5 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human u-Plasminogen Activator/Urokinase (Catalog # 1310-SE) to immobilized Recombinant Mouse uPAR Fc Chimera (Catalog # 531-PA) coated at 5 µg/mL (100 µL/well). At 25 μg/mL, this antibody will block >90% of the binding.

 
CyTOF-reported
Lujan, E. et al. (2015) Nature 521: 352. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of uPAR antibody in Mouse Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of uPAR in Mouse Blood Monocytes by Flow Cytometry. Mouse whole blood monocytes were stained with Rat Anti-Mouse uPAR Monoclonal Antibody (Catalog # MAB531, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by anti-Rat IgG PE-conjugated Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: uPAR

The urokinase-type plasminogen activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a
single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. Mouse uPAR-1/Fc cDNA encodes a 327 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide, seven potential N-linked glycosylation sites and a C-terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is highly species-specific. Human uPA binds rmuPAR at a lower affinity compared to rhuPAR.

References
  1. Dear, A.E. and R.L. Medcalf (1998) Eur. J. Biochemistry 252:185.
Long Name
Urokinase-type Plasminogen Activator Receptor
Entrez Gene IDs
5329 (Human); 18793 (Mouse); 102139334 (Cynomolgus Monkey)
Alternate Names
CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3

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Citations for Mouse uPAR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. SuPAR mediates viral response proteinuria by rapidly changing podocyte function
    Authors: Wei, C;Datta, PK;Siegerist, F;Li, J;Yashwanth, S;Koh, KH;Kriho, NW;Ismail, A;Luo, S;Fischer, T;Amber, KT;Cimbaluk, D;Landay, A;Endlich, N;Rappaport, J;Michigan Medicine COVID−19 Investigators, ;Hayek, SS;Reiser, J;
    Nature communications
  2. Urokinase-Type Plasminogen Activator Receptor Regulates Prosurvival and Angiogenic Properties of Cardiac Mesenchymal Stromal Cells
    Authors: Dergilev, K;Tsokolaeva, Z;Goltseva, Y;Beloglazova, I;Ratner, E;Parfyonova, Y;
    International journal of molecular sciences
    Species: Transgenic Mouse, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Targeting uPA-uPAR interaction to improve intestinal epithelial barrier integrity in inflammatory bowel disease
    Authors: Y Cheng, TR Hall, X Xu, I Yung, D Souza, J Zheng, F Schiele, M Hoffmann, ML Mbow, JP Garnett, J Li
    EBioMedicine, 2021-12-18;75(0):103758.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. TLR4 Response to LPS Is Reinforced by Urokinase Receptor
    Authors: Yulia Kiyan, Sergey Tkachuk, Song Rong, Anna Gorrasi, Pia Ragno, Inna Dumler et al.
    Frontiers in Immunology
  5. Senolytic CAR T cells reverse senescence-associated pathologies
    Authors: C Amor, J Feucht, J Leibold, YJ Ho, C Zhu, D Alonso-Cur, J Mansilla-S, JA Boyer, X Li, T Giavridis, A Kulick, S Houlihan, E Peerschke, SL Friedman, V Ponomarev, A Piersigill, M Sadelain, SW Lowe
    Nature, 2020-06-17;0(0):.
    Species: Mouse
    Sample Types: Antibody
    Applications: Mass Spectrometry
  6. FAP promotes immunosuppression by cancer-associated fibroblasts in the tumor microenvironment via STAT3-CCL2 signaling
    Cancer Res, 2016-05-23;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  7. Progression of Osteosarcoma from a Non-Metastatic to a Metastatic Phenotype Is Causally Associated with Activation of an Autocrine and Paracrine uPA Axis.
    Authors: Endo-Munoz L, Cai N, Cumming A, Macklin R, Merida de Long L, Topkas E, Mukhopadhyay P, Hill M, Saunders N
    PLoS ONE, 2015-08-28;10(8):e0133592.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Administration of Recombinant Soluble Urokinase Receptor Per Se Is Not Sufficient to Induce Podocyte Alterations and Proteinuria in Mice
    Authors: Dominique Cathelin, Sandrine Placier, Michael Ploug, Marie-Christine Verpont, Sophie Vandermeersch, Yosu Luque et al.
    Journal of the American Society of Nephrology
  9. Loss of urokinase receptor sensitizes cells to DNA damage and delays DNA repair.
    Authors: Narayanaswamy, Pavan B, Hodjat, Mahshid, Haller, Hermann, Dumler, Inna, Kiyan, Yulia
    PLoS ONE, 2014-07-02;9(7):e101529.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Urokinase-type plasminogen activator receptor (uPAR) augments brain damage in a murine model of ischemic stroke.
    Authors: Nagai N, Okada K, Kawao N, Ishida C, Ueshima S, Collen D, Matsuo O
    Neurosci. Lett., 2007-12-08;432(1):46-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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