Home / ULBP-1 / Mouse ULBP-1/MULT-1 PE-conjugated Antibody

Mouse ULBP-1/MULT-1 PE-conjugated Antibody

Catalog #: FAB2588P
6 Citations Datasheet / COA / SDS
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FAB2588P
Detection of ULBP‑1 in A20 Mouse Cell Line by Flow Cytometry.
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Citations (6)
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Mouse ULBP-1/MULT-1 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ULBP‑1 /MULT-1 in flow cytometry.
Source
Monoclonal Rat IgG2A Clone # 237104
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with mouse ULBP‑1 /MULT-1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ULBP-1 antibody in A20 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of ULBP‑1 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse ULBP-1 /MULT-1 PE-conjugated Monoclonal Antibody (Catalog # FAB2588P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: ULBP-1

ULBP-1, also known as MULT-1 (Mouse UL16-Binding Protein-like Transcript 1), is a 53 kDa, MHC Class I-like molecule that belongs to the mouse family of NKG2D ligands (1‑4). It is a type I transmembrane glycoprotein that is synthesized as a 334 amino acid (aa) precursor. It contains a 25 aa signal sequence, a 186 aa extracellular region, a 19 aa transmembrane segment and a 104 aa cytoplasmic tail (2). The extracellular region contains an alpha 1 and alpha 2 like domain with two intrachain disulfide bonds. ULBP-1 is distantly related to other human and mouse NKG2D ligands, and more distantly related to the MHC class I proteins (3). Unlike most NKG2D ligands, transcripts for ULBP-1 have been detected in a wide variety of mouse tissues and tumor cells lines (3). The receptor for ULBP-1 is NKG2D, a 35 kDa C-type lectin that is found on mouse NK cells, activated CD8+ T cells, epidermal gamma δ T cells, and activated macrophages (1, 5, 6, 7). Recombinant ULBP-1 protein binds to NKG2D with high affinity (KD = 6 nM) (2). Although an activating receptor, general cellular responses to NKG2D ligation depend upon the isoform of NKG2D and the cell type (5). Exposure to immobilized ULBP-1 or ULBP-1-transfected cells elicits IFN-gamma production by NK cells (3). Ectopic expression of ULBP-1 on the RMA mouse tumor cell line leads to tumor rejection in syngeneic mice (3).

References
  1. Raulet, D.H. (2003) Nat. Rev. Immunol. 3:781.
  2. Carayannopoulos, L. et al. (2002) J. Immunol. 169:4079.
  3. Diefenbach, A. et al. (2003) Eur. J. Immunol. 33:381.
  4. Krmpotic, A. et al. (2005) J. Exp. Med. 201:211.
  5. Diefenbach, A. et al. (2002) Nat. Immunol. 3:1142.
  6. Ho, E.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6320.
  7. Carayannopoulos, L.N. et al. (2002) Eur. J. Immunol. 32:597.
Long Name
UL16 Binding Protein-1
Entrez Gene IDs
80329 (Human); 77777 (Mouse)
Alternate Names
ALCAN-beta; MULT-1; N2DL1; N2DL-1; NKG2D ligand 1; NKG2DL; NKG2DL1; RAET1I; RAET1Ialcan-beta; Retinoic acid early transcript 1I; UL16 binding protein 1; UL16-binding protein 1; ULBP1; ULBP-1

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Citations for Mouse ULBP-1/MULT-1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Upregulation of NKG2D ligands impairs hematopoietic stem cell function in Fanconi anemia
    Authors: José A. Casado, Antonio Valeri, Rebeca Sanchez-Domínguez, Paula Vela, Andrea López, Susana Navarro et al.
    Journal of Clinical Investigation
  2. Epigenetic silencing by SETDB1 suppresses tumour intrinsic immunogenicity
    Authors: GK Griffin, J Wu, A Iracheta-V, JC Patti, J Hsu, T Davis, D Dele-Oni, PP Du, AG Halawi, JJ Ishizuka, SY Kim, S Klaeger, NH Knudsen, BC Miller, TH Nguyen, KE Olander, M Papanastas, S Rachimi, EJ Robitschek, EM Schneider, MD Yeary, MD Zimmer, JD Jaffe, SA Carr, JG Doench, WN Haining, KB Yates, RT Manguso, BE Bernstein
    Nature, 2021-05-05;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. NKG2D is Required for Regulation of Lung Pathology and Dendritic Cell Function Following RSV Infection
    Authors: H Liu, AR Osterburg, J Flury, S Huang, FX McCormack, SA Cormier, MT Borchers
    J. Infect. Dis., 2018-10-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. NKG2D-NKG2D Ligand Interaction Inhibits the Outgrowth of Naturally Arising Low-Grade B Cell Lymphoma In Vivo
    J Immunol, 2016-05-02;196(11):4805-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Toll-Like receptor 2 (TLR2) and TLR9 play opposing roles in host innate immunity against Salmonella enterica serovar Typhimurium infection.
    Authors: Zhan R, Han Q, Zhang C, Tian Z, Zhang J
    Infect Immun, 2015-02-09;83(4):1641-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Role of CC chemokine receptor 4 in natural killer cell activation during acute cigarette smoke exposure.
    Authors: Stolberg V, Martin B, Mancuso P, Olszewski M, Freeman C, Curtis J, Chensue S
    Am J Pathol, 2013-12-09;184(2):454-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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