Mouse TLR6 PE-conjugated Antibody Summary
Phe39-Thr806
Accession # BAA78632
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of TLR6 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TLR6 PE-conjugated Monoclonal Antibody (Catalog # FAB1533P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
Background: TLR6
The Toll-like family of molecules are a group of integral membrane proteins that serve as pattern recognition receptors for microbial pathogens. There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (1‑4). All Toll-like receptors (TLRs) are type I transmembrane (TM) proteins that exist either in the plasma membrane or in the membranes of endosomal structures (where they bind intracellular microbial nucleic acids). All TLRs also contain a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL-1 receptor (TIR) domain. The mouse TLR6 cDNA encodes a 795 amino acid (aa) precursor that includes a 27 aa signal sequence, a 557 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 190 aa cytoplasmic domain. The ECD contains 14 Leu-rich repeats, and the cytoplasmic region contains one TIR domain (5). Within the ECD, mouse TLR6 shares 59% aa sequence identity with mouse TLR1 and 20‑27% aa sequence identitity with mouse TLR2, -3, -4, -5, -7, -8, -9, -11, -12, and -13. It shares 71%, 72%, and 86% aa sequence identity with bovine, human, and rat TLR6, respectively. TLR6 is expressed on the cell surface of macrophages, monocytes, neutrophils, and dermal endothelial cells in ligand-independent association with TLR2 (6‑9). TLR2 also associates with TLR1, a functional complex with specificity for distinct but related microbial ligands (6‑8). TLR6 and TLR2 cooperate in the recognition of acylated bacterial and mycoplasma lipopeptides, peptidoglycan, and glycosylphosphatidylinositols (7‑14). The cytoplasmic TIR domain is necessary and sufficient to initiate signal transduction which leads to activation of NF kappa B (7, 15).
- Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
- Roeder, A. et al. (2004) Med. Mycol. 42:485.
- Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
- Wetzler, L.M. (2003) Vaccine 21:S55.
- Takeuchi, O. et al. (1999) Gene 231:59.
- Hajjar, A.M. et al. (2001) J. Immunol. 166:15.
- Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:13766.
- Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
- Nakao, Y. et al. (2005) J. Immunol. 174:1566.
- Bulut, Y. et al. (2001) J. Immunol. 167:987.
- Takeuchi, O. et al. (2001) Int. Immunol. 13:933.
- Morr, M. et al. (2002) Eur. J. Immunol. 32:3337.
- Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
- Omueti, K.O. et al. (2005) J. Biol. Chem. 280:36616.
- Nishiya, T. and A.L. DeFranco (2004) J. Biol. Chem. 279:19008.
Product Datasheets
Citations for Mouse TLR6 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Divergent Immune Responses to Minor Bovine Mastitis-Causing Pathogens
Authors: Srithanasuwan, A;Pangprasit, N;Mektrirat, R;Suriyasathaporn, W;Chuammitri, P;
Veterinary sciences
Species: Bovine
Sample Types: Whole Cells
Applications: Flow Cytometry -
A lymphatic route for a hyperbranched heteroglycan from Radix Astragali to trigger immune responses after oral dosing
Authors: Q Zhang, L Li, S Hao, M Liu, C Huo, J Wu, H Liu, W Bao, H Zheng, Z Li, H Cheng, H Fung, T Wong, P Leung, S Wang, T Li, G Zhang, M Li, Z Zhao, W Jia, Z Bian, T Mitchison, J Zhang, A Lyu, Q Han, H Sun
Carbohydrate polymers, 2022-05-27;292(0):119653.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
TLR induced IL-27 plays host-protective role against B16BL6 melanoma in C57BL/6 mice
Authors: A Patidar, S Selvaraj, M Chakravart, I Guha, A Bhuniya, S Bera, S Dhar, K Roy, R Baral, D Chattopadh, C Pal, B Saha
Cytokine, 2022-04-15;154(0):155871.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Induction of Foxp3 and activation of Tregs by HSP gp96 for treatment of autoimmune diseases
Authors: Yuxiu Xu, Erlong Liu, Xialin Xie, Jiuru Wang, Huaguo Zheng, Ying Ju et al.
iScience
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