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Mouse Thrombomodulin/BDCA-3 Antibody

Catalog #: AF3894
14 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3894
AF3894-SP
Thrombomodulin/BDCA‑3 in bEnd.3 Mouse Cell Line.
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Citations (14)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Thrombomodulin/BDCA-3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Thrombomodulin/BDCA-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
2% cross‑reactivity with recombinant human Thrombomodulin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Thrombomodulin/BDCA-3
Leu17-Ser517
Accession # P15306
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Thrombomodulin/BDCA-3 (Catalog # 3894-PA)
Flow Cytometry
2.5 µg/106 cells
bEnd.3 mouse endothelioma cell line
Immunohistochemistry
1-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Thrombomodulin/BDCA-3 (Catalog # 3894-PA), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Thrombomodulin/BDCA-3 antibody in bEnd by Immunocytochemistry (ICC).3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Thrombomodulin/BDCA‑3 in bEnd.3 Mouse Cell Line. Thrombomodulin/BDCA-3 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse Thrombomodulin/BDCA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3894) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Thrombomodulin/BDCA-3 antibody in Human Kidney Tissue by Immunohistochemistry (IHC-P). View Larger

Thrombomodulin/BDCA-3 in Human Kidney Tissue. Thrombomodulin/BDCA-3 was detected in immersion fixed paraffin-embedded sections of human kidney tissue using Goat Anti-Mouse Thrombomodulin/BDCA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3894) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface on endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombomodulin/BDCA-3

Encoded by the THBD gene, Thrombomodulin is also known as CD141 antigen. The deduced amino acid sequence of mouse THBD predicts a signal peptide (aa 1 to 16) and a mature chain (aa 17 to 577) that consists of the following domains: C-type lectin (aa 31 to 167), EGF-like (aa 240 to 280, aa 283 to 323, aa 324 to 362, aa 364 to 404, aa 405 to 439, and aa 440 to 480), transmembrane (aa 518 to 541) and cytoplasmic (aa 542 to 577) (1). The R&D Systems recombinant mouse THBD consists of aa 17 to 517, corresponding to the extracellular portion of the type I membrane protein.

Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis (2‑4). It functions as a cell surface receptor and an essential cofactor for active thrombin, which in turn activates protein C and thrombin-activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2). Activated protein C (APC), facilitated by protein S, degrades coagulation factors Va and VIIIa, which are required for thrombin activation. Activated CPB2 cleaves basic C-terminal amino acid residues of its substrates, including fibrin, preventing the conversion of plasminogen to plasmin. In addition, THBD gene polymorphisims are associated with human disease and THBD plays a role in thrombosis, stroke, arteriosclerosis, and cancer (5). For example, increased serum levels of THBD, due to protease cleavage, have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis (6).

References
  1. Dittman, W.A. and P.W. Majerus (1989) Nucleic Acids Res. 17:802.
  2. Van de Wouwer, M. et al. (2004) Arterioscler. Thromb. Vasc. 24:1374.
  3. Wu, K.K. et al. (2000) Ann Med. 32:73.
  4. Li, Y.H. et al. (2006) Cardiovasc. Hematol. Agents Med. Chem. 4:183.
  5. Weiler, H. and B.H. Isermann (2003) J. Thromb. Haemost. 1:1515.
  6. Califano, F. et al. (2000) Eur. Rev. Med. Pharmacol. Sci. 4:59.
Entrez Gene IDs
7056 (Human); 21824 (Mouse); 83580 (Rat)
Alternate Names
BDCA3; BDCA-3; CD141 antigen; CD141; Fetomodulin; THBD; THRM; Thrombomodulin; TM

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Citations for Mouse Thrombomodulin/BDCA-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Variable phenotypic penetrance of thrombosis in adult mice after tissue-selective and temporally controlled Thbd gene inactivation
    Authors: Thijs E. van Mens, Hai-Po H. Liang, Sreemanti Basu, Irene Hernandez, Mark Zogg, Jennifer May et al.
    Blood Advances
  2. Endothelial Progenitors Exist within the Kidney and Lung Mesenchyme
    Authors: Sunder Sims-Lucas, Caitlin Schaefer, Daniel Bushnell, Jacqueline Ho, Alison Logar, Edward Prochownik et al.
    PLoS ONE
  3. The Adult Murine Intestine is Dependent on Constitutive Laminin-gamma1 Synthesis
    Authors: B Fields, A DeLaForest, M Zogg, J May, C Hagen, K Komnick, J Wieser, A Lundberg, H Weiler, MA Battle, KS Carlson
    Sci Rep, 2019-12-17;9(1):19303.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Neferine Protects Endothelial Glycocalyx via Mitochondrial ROS in Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome
    Authors: XY Liu, HX Xu, JK Li, D Zhang, XH Ma, LN Huang, JH Lü, XZ Wang
    Front Physiol, 2018-02-22;9(0):102.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Lack of ABCG2 Leads to Biventricular Dysfunction and Remodeling in Response to Hypoxia
    Authors: BM Nagy, C Nagaraj, B Egemnazaro, G Kwapiszews, RE Stauber, A Avian, H Olschewski, A Olschewski
    Front Physiol, 2017-02-21;8(0):98.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Vascular immunotargeting to endothelial determinant ICAM-1 enables optimal partnering of recombinant scFv-thrombomodulin fusion with endogenous cofactor.
    Authors: Greineder, Colin F, Chacko, Ann-Mari, Zaytsev, Sergei, Zern, Blaine J, Carnemolla, Ronald, Hood, Elizabet, Han, Jingyan, Ding, Bi-Sen, Esmon, Charles, Muzykantov, Vladimir
    PLoS ONE, 2013-11-14;8(11):e80110.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  7. The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis.
    Nat. Med., 2012-07-22;18(0):1217.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P
  8. Schistosomiasis-induced experimental pulmonary hypertension: role of interleukin-13 signaling.
    Authors: Graham BB, Mentink-Kane MM, El-Haddad H
    Am. J. Pathol., 2010-07-29;177(3):1549-61.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Both UFH and NAH alleviate shedding of endothelial glycocalyx and coagulopathy in LPS‑induced sepsis
    Authors: Xiao Huang, Shasha Han, Xiangyong Liu, Tao Wang, Haixiao Xu, Bingyan Xia et al.
    Experimental and Therapeutic Medicine
  10. Single-cell transcriptomics reveals zone-specific alterations of liver sinusoidal endothelial cells in cirrhosis
    Authors: T Su, Y Yang, S Lai, J Jeong, Y Jung, M McConnell, T Utsumi, Y Iwakiri
    Cellular and Molecular Gastroenterology and Hepatology, 2020-12-16;0(0):.
  11. Graft protective effect and induction of CD4+Foxp3+ cell by Thrombomodulin on allograft arteriosclerosis in mice
    Authors: Enzhi Yin, Shigefumi Matsuyama, Masateru Uchiyama, Kento Kawai, Masanori Niimi
    Journal of Cardiothoracic Surgery
  12. Haptoglobin-2 variant increases susceptibility to acute respiratory distress syndrome during sepsis
    Authors: V. Eric Kerchberger, Julie A. Bastarache, Ciara M. Shaver, Hiromasa Nagata, J. Brennan McNeil, Stuart R. Landstreet et al.
    JCI Insight
  13. Age-associated pro-inflammatory adaptations of the mouse thoracic aorta
    Authors: Bianca Hemmeryckx, Marc F. Hoylaerts, Eveline Deloose, Cor E. Van Hove, Paul Fransen, Hidde Bult et al.
    Thrombosis and Haemostasis
  14. Role of thrombomodulin expression on hematopoietic stem cells
    Authors: Sreemanti Basu, Hai Po Helena Liang, Irene Hernandez, Mark Zogg, British Fields, Jennifer May et al.
    Journal of Thrombosis and Haemostasis

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