Mouse TGF-beta 2 Antibody

Catalog #: MAB7346
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7346-SP
MAB7346-500
MAB7346-100

TGF‑ beta 2 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by Mouse TGF‑ beta 2 Antibody.

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Citations (5)

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Mouse TGF-beta 2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TGF‑ beta 2 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) TGF-beta 2 and rhTGF-beta 3 is observed.

Source

Monoclonal Rat IgG_2B Clone # 771213

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse TGF‑ beta 2
Ala303-Ser414
Accession # P27090

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize TGF‑ beta 2 inhibition of IL-4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND₅₀) is typically 0.15-0.75 ug/mL in the presence of 5 ng/mL Recombinant Mouse TGF‑ beta 2 and 7.5 ng/mL Recombinant Mouse IL-4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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TGF‑ beta 2 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by Mouse TGF‑ beta 2 Antibody. Recombinant Mouse TGF-beta 2 (Catalog # 7346-B2) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Mouse TGF-beta 2 (5 ng/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse TGF-beta 2 Monoclonal Antibody (Catalog # MAB7346). The ND₅₀ is typically 0.15-0.75 ug/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 2

TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cysteine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems. Mouse TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 283 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 2. Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 2 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature mouse TGF-beta 2 shares 100% aa identity with rat TGF-beta 2, and 97% aa identity with human, porcine, canine, equine and bovine TGF-beta 2. It demonstrates cross-species activity. In most cells, TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II ser/thr kinase receptor termed TGF-beta RII, which then phosphorylates and activates another ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription. In bone -related cells, however, TGF-beta 2 also signals through TGF-beta RIIB (a splice variant of TGF-beta RII), independently of TGF-beta RIII. Use of other signaling pathways that are Smad-independent allows for disparate actions observed in response to TGF-beta in different contexts.

Long Name

Transforming Growth Factor beta 2

Entrez Gene IDs

7042 (Human); 21808 (Mouse); 397084 (Porcine)

Alternate Names

BSC-1 cell growth inhibitor; cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; MGC116892; polyergin; TGFB2; TGFbeta 2; TGF-beta 2; TGF-beta2; TGF-beta-2; transforming growth factor beta-2; transforming growth factor, beta 2

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Citations for Mouse TGF-beta 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Elevated TGF Î²2 serum levels in Emery-Dreifuss muscular dystrophy: implications for myocyte and tenocyte differentiation and fibrogenic processes
Authors: P Bernasconi, N Carboni, G Ricci, G Siciliano, L Politano, L Maggi, T Mongini, L Vercelli, C Rodolico, E Biagini, G Boriani, L Ruggiero, L Santoro, E Schena, S Prencipe, C Evangelist, E Pegoraro, L Morandi, M Columbaro, C Lanzuolo, P Sabatelli, P Cavalcante, C Cappellett, G Bonne, A Muchir, G Lattanzi
Nucleus, 2018-01-01;0(0):1-24.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
CYP4A in tumor-associated macrophages promotes pre-metastatic niche formation and metastasis
Authors: XW Chen, TJ Yu, J Zhang, Y Li, HL Chen, GF Yang, W Yu, YZ Liu, XX Liu, CF Duan, HL Tang, M Qiu, CL Wang, H Zheng, J Yue, AM Guo, J Yang
Oncogene, 2017-05-08;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Functional Assay
Immunomodulatory cross-talk between conjunctival goblet cells and dendritic cells.
Authors: Contreras-Ruiz L, Masli S
PLoS ONE, 2015-03-20;10(3):e0120284.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Neutralization
Neutrophils increase or reduce parasite burden in Trypanosoma cruzi-infected macrophages, depending on host strain: role of neutrophil elastase.
Authors: Luna-Gomes T, Filardy A, Rocha J, Decote-Ricardo D, LaRocque-de-Freitas I, Morrot A, Bozza P, Castro-Faria-Neto H, DosReis G, Nunes M, Freire-de-Lima C
PLoS ONE, 2014-03-05;9(3):e90582.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Endothelial lineage differentiation from induced pluripotent stem cells is regulated by microRNA-21 and transforming growth factor beta2 (TGF-beta2) pathways.
Authors: Di Bernardini E, Campagnolo P, Margariti A, Zampetaki A, Karamariti E, Hu Y, Xu Q
J Biol Chem, 2013-12-19;289(6):3383-93.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization