Mouse Testican 3/SPOCK3 Antibody

Catalog # Availability Size / Price Qty
MAB2346
MAB2346-SP
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Mouse Testican 3/SPOCK3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Testican 3/SPOCK3 in ELISAs. In sandwich immunoassays, less than 0.25% cross-reactivity with recombinant human (rh) Testican 1 is observed and no cross-reactivity with rhTestican 2 is observed.
Source
Monoclonal Rat IgG1 Clone # 330403
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Testican 3/SPOCK3
Val26-Ile436
Accession # Q8BKV0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample

Mouse Testican 3/SPOCK Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Testican 3/SPOCK3 Biotinylated Antibody (Catalog # BAF2346)

Standard: Recombinant Mouse Testican 3/SPOCK3 Protein, CF (Catalog # 2346-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Testican 3/SPOCK3

Testican 3 encoded by the SPOCK3 gene is a proteoglycan expressed in brain (1). Human and mouse Testican 3 share 90% amino acid sequence identity, indicating a conserved function (2, 3). Testican 3 contains Ca2+-binding domain and the C-terminal acidic domain with putative glycosaminoglycan attachment sites. In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. The N-terminal region, which is unique to Testicans, is responsible for the inhibition of Testican 3 towards MMP-14/MT1-MMP activation of MMP-2 (1). The thyropin domain and the follistatin-like domain with a six cysteine Kazal-like motif may inhibit cysteine and serine proteases, respectively (4). A spliced variant designated as N-Tes contains the N-terminal unique region, the follistatin-like domain and the Ca2+-binding domain, but lacks the C-terminal thyropin domain and the acidic domain (1).

References
  1. Nakada, M. et al. (2001) Cancer Res. 61:8896.
  2. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
  3. Okazaki, Y. et al. (2002) Nature 420:563.
  4. Alliel, P.M. et al. (1993) Eur. J. Biochem. 214:347.
Entrez Gene IDs
50859 (Human); 72902 (Mouse)
Alternate Names
CWCV, and Kazal-like domains proteoglycan 3; HSAJ1454; sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3; SPOCK3; TES-3; Testican 3; TICN3

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