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Mouse Syndecan-3 Antibody

Catalog #: AF2734
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2734
AF2734-SP
Syndecan‑3 in Mouse Kidney.
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Citations (2)
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Mouse Syndecan-3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Syndecan-3 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Syndecan-3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Syndecan-3
Ala45-Glu384
Accession # Q64519
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Syndecan‑3
Immunohistochemistry
0.5-15 µg/mL
Paraffin-embedded sections of mouse kidney

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Syndecan‑3 antibody in Mouse Kidney by Immunohistochemistry (IHC-P). View Larger

Syndecan‑3 in Mouse Kidney. Syndecan‑3 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse Syndecan‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2734) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane of epithelial cells in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syndecan-3

Mouse Syndecan-3 (also known as N-Syndecan) is a variably glycosylated type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 442 amino acid (aa) precursor with a 45 aa signal sequence, a 339 aa extracellular domain (ECD), a 25 aa TM segment and a 33 aa cytoplasmic region. It presumably exists as a dimer. Heparan sulfate modification of the ECD yields a native molecular weight that exceeds 200 kDa. Soluble forms generated by proteolytic processing are suggested to exist. Syndecan-3 is a musculoskeletal and neuronal molecule which mediates neurite outgrowth and myoblast differentiation. Mouse Syndecan-3 ECD shares 95% and 81% aa sequence identity with rat and human Syndecan-3 ECD, respectively.

Entrez Gene IDs
9672 (Human); 20970 (Mouse)
Alternate Names
KIAA0468; N-Syndecan; SDC3; SDCN; SYND3syndecan proteoglycan 3; syndecan 3 (N-syndecan); syndecan 3; syndecan neural type; Syndecan3; Syndecan-3

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Citations for Mouse Syndecan-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A heparan-sulfate-bearing syndecan-1 glycoform is a distinct surface marker for intra-tumoral myeloid-derived suppressor cells
    Authors: Thomas Welte, Junhua Mai, Zhe Zhang, Shaohui Tian, Guodong Zhang, Yitian Xu et al.
    iScience
  2. Heparan sulfate subdomains that are degraded by Sulf accumulate in cerebral amyloid beta plaques of Alzheimer's disease: evidence from mouse models and patients.
    Authors: Hosono-Fukao T, Ohtake-Niimi S, Hoshino H, Britschgi M, Akatsu H, Hossain MM, Nishitsuji K, van Kuppevelt TH, Kimata K, Michikawa M, Wyss-Coray T, Uchimura K
    Am. J. Pathol., 2012-03-17;180(5):2056-67.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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