Mouse SIGIRR Antibody

Catalog #: MAB1092 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1092
MAB1092-SP

All SIGIRR Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse SIGIRR Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse SIGIRR in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) IL‑1 R1, rmIL-1 R2, rhIL-1 RAcP, recombinant human (rh) IL-1 R4, rmIL-18 R, recombinant rat IL-1 R6, rmIL-1 R7, rhIL-1 R8, rhIL-1 R9, or rhSIGIRR.

Source

Monoclonal Rat IgG₁ Clone # 161917

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SIGIRR
Met1-His117
Accession # Q9JLZ8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse SIGIRR Fc Chimera (Catalog # 992-SG)
under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIGIRR

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL-18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL1RAPL1), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Five of the IL-1 R family members (1, 2, 4, 5, and 6) are clustered and localized to chromosome 2. SIGIRR (single Ig domain containing IL-1 receptor-related molecule) is a subtype of the IL-1 R family that differs from the other nine members by having only one Ig domain in its extracellular region. The sequence of human SIGIRR predicts a 410 amino acid (aa) residue transmembrane glycoprotein that lacks signal peptide and contains a 118 aa single Ig extracellular domain, a transmembrane region and a 268 aa cytoplasmic tail with a TIR domain. The cytoplasmic tail of SIGIRR contains a C-terminal extention beyond the TIR domain which is also found in IL1RAPL1, IL-1 R9, and Toll-like receptor family members but absent in other IL-1 receptor family members. SIGIRR is widely expressed and is present in all cells and tissues examined. Mouse and human SIGIRR share 82% amino acid sequence identity. The ligand and signaling mechanism for SIGIRR has not been identified.

References

Thomassen, E. et al. (1999) Cytokine 11:389.

Long Name

Single lg IL-1 Related Protein

Entrez Gene IDs

59307 (Human); 24058 (Mouse)

Alternate Names

IL-1R8; SIGIRR; single Ig IL-1-related receptor; Single Ig IL-1R-related molecule; single immunoglobulin and toll-interleukin 1 receptor (TIR) domain; single immunoglobulin domain IL1R1 related; Single immunoglobulin domain-containing IL1R-related protein; TIR8; TIR8MGC110992; Toll/interleukin-1 receptor 8