Mouse Serpin E1/PAI-1 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF3828
Detection of Mouse Serpin E1/PAI‑1 by Western Blot.
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Mouse Serpin E1/PAI-1 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Serpin E1/PAI‑1 in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Serpin E1 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) Serpin E2 and rmSerpin I1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Serpin E1/PAI‑1
Leu24-Ser402
Accession # AAH54091
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Serpin E1/PAI-1 antibody by Western Blot. View Larger

Detection of Mouse Serpin E1/PAI‑1 by Western Blot. Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3828) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. A specific band was detected for Serpin E1/PAI-1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin E1/PAI-1

Serpin E1, also known as Plasminogen Activator Inhibitor-1 (PAI-1), is the principal inhibitor of urokinase-type and tissue-type plasminogen activators. It plays a major role in many processes such as angiogenesis, tumor invasion, and obesity. The amino acid sequence of mouse PAI-1 is 89%, 79% and 76% identical to that of rat, human/chimpanzee and dog.

Long Name
Plasminogen Activator Inhibitor
Entrez Gene IDs
5054 (Human); 18787 (Mouse)
Alternate Names
Endothelial plasminogen activator inhibitor; Nexin; PAI1; PAI-1; PAI1PAI-1; PAISerpin E1; PLANH1; PLANH1plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

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