Mouse Sca-1/Ly6 Alexa Fluor® 488-conjugated Antibody Summary
Leu27-Gly119
Accession # CAA28351
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Sca‑1/Ly6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit APC-conjugated Monoclonal Antibody (Catalog # FAB1356A) and either (A) Rat Anti-Mouse Sca-1/Ly6 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1226G) or (B) Rat IgG2AAlexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
Background: Sca-1/Ly6
Stem Cell Antigen-1 (Sca-1) is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly-6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non-hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12). Over amino acids (aa) 27-119, mouse Sca-1 shares 58% aa identity with rat Ly6B.
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- Spangrude, G.I. et al. (1988) Science 241:58.
- Spangrude, G.I. et al. (1993) Blood 82:3327.
- Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
- Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
- Yamamoto, Y. et al. (1996) Blood 88:445.
- Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
- Welm, B.E. et al. (2002) Dev. Biol. 245:42.
- Codias, E.K. et al. (1990) J. Immunol. 144:2197.
- Malek, T.R. et al. (1986) J. Exp. Med. 164:709.
- Codias, E.K. et al. (1990) J. Immunol. 145:1407.
- Flood, P.M. et al. (1990) J. Exp. Med. 172:115.
Product Datasheets
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