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Mouse Reg2 Antibody

Catalog #: MAB2098
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2098
MAB2098-SP
Detection of Mouse Reg2 by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Reg2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Reg2 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) Reg1, recombinant rat (rr) Reg2, rmReg3A, recombinant human Reg4, or rrReg4.
Source
Monoclonal Rat IgG2A Clone # 239237
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Reg2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Reg2 antibody by Western Blot. View Larger

Detection of Mouse Reg2 by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line, mouse small intestine and pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Reg2 Monoclonal Antibody (Catalog # MAB2098) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reg2

Reg2 is a secreted C-type lectin that is expressed in regenerating pancreatic islets and stimulates the growth of pancreatic beta cells.

Long Name
Regenerating Islet-derived 2
Entrez Gene IDs
19693 (Mouse)
Alternate Names
Reg2; regenerating islet-derived 2; RegII

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Citations for Mouse Reg2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Role and mechanism of REG2 depletion in insulin secretion augmented by glutathione peroxidase-1 overproduction
    Authors: X Yan, Z Zhao, J Weaver, T Sun, JW Yun, CA Roneker, F Hu, NM Doliba, CCW McCormick, MZ Vatamaniuk, XG Lei
    Redox Biology, 2022-08-30;56(0):102457.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. Adjuvant immunotherapy increases beta cell regenerative factor Reg2 in the pancreas of diabetic mice.
    Authors: Huszarik K, Wright B, Keller C
    J. Immunol., 2010-09-27;185(9):5120-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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