Home / PRAT4B / Mouse/Rat PRAT4B Antibody

Mouse/Rat PRAT4B Antibody

Catalog #: AF5015
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5015
AF5015-SP
Detection of Mouse and Rat PRAT4B by Western Blot.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat PRAT4B Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat PRAT4B in Western blots.  In direct ELISAs, less than 5% cross-reactivity with recombinant human PRAT4B and recombinant mouse PRAT4A is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derive recombinant mouse PRAT4B
Thr30-Leu245
Accession # Q8BQ47
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat PRAT4B antibody by Western Blot. View Larger

Detection of Mouse and Rat PRAT4B by Western Blot. Western blot shows lysates of rat lung tissue and mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat PRAT4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5015) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PRAT4B at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of PRAT4B antibody in RAW264.7 Mouse Cell Line antibody by Flow Cytometry View Larger

Detection of PRAT4B in RAW264.7 Mouse Cell Line by Flow Cytometry RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse/Rat PRAT4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5015, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PRAT4B

PRAT4B (protein associated with TLR4-B; also Cnpy4, protein canopy homolog 4) is a member of the canopy family of proteins. It is expressed by diverse cell types, including macrophages, fibroblasts and B cells. PRAT4B appears to be both secreted, and retained internally, where it may stabilize immature TLR protein expression in the ER, and impact its appearance on the cell surface. Mouse PRAT4B is 245 amino acids (aa) in length. It contains a 27 aa signal sequence plus one coiled-coil region (aa 209 - 237). There is one potential splice variant that shows a 13 aa substitution for the C-terminal nine amino acids. Over aa 30 - 245, mouse PRAT4B is 95% and 87% aa identical to rat and human PRAT4B, respectively.

Long Name
Protein Associated with Toll-like Receptor 4B
Entrez Gene IDs
245812 (Human); 66455 (Mouse); 363886 (Rat)
Alternate Names
canopy 4 homolog (zebrafish); CNPY4; MGC40499PRAT4BPRotein Associated with Tlr4; PRAT4B; protein canopy homolog 4

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Citation for Mouse/Rat PRAT4B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CNPY4 inhibits the Hedgehog pathway by modulating membrane sterol lipids
    Authors: M Lo, A Sharir, MD Paul, H Torosyan, C Agnew, A Li, C Neben, P Marangoni, L Xu, DR Raleigh, N Jura, OD Klein
    Nature Communications, 2022-05-03;13(1):2407.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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