Mouse/Rat DARC APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6695A-100UG
Detection of DARC in Mouse Splenocytes by Flow Cytometry.
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Citations (1)
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Mouse/Rat DARC APC-conjugated Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat DARC in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse DARC
Met1-Pro61, Ala115-Cys127, Ser186-Lys205, Tyr264-Asn285
Accession # NP_034175
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of DARC antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of DARC in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse TER-119 PE-conjugated Monoclonal Antibody (Catalog # FAB1125P) and either (A) Sheep Anti-Mouse/Rat DARC APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6695A) or (B) Normal Sheep IgG Allophycocyanin Control (Catalog # IC016A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of DARC antibody in HEK293 Human Cell Line Transfected with Mouse DARC and eGFP antibody by Flow Cytometry. View Larger

Detection of DARC in HEK293 Human Cell Line Transfected with Mouse DARC and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse DARC and eGFP was stained with either (A) Sheep Anti-Mouse/Rat DARC APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6695A) or (B) Normal Sheep IgG Allophycocyanin Control (Catalog # IC016A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: DARC

DARC (Duffy Antigen Receptor for Chemokines; also CD234) is a 40-46 kDa glycoprotein member of the Duffy family of silent heptahelical chemokine receptors. It is expressed in liver and on select neurons, erythrocytes and the endothelium of postcapillary venules. Unlike traditional chemokine receptors, DARC cannot signal through G-proteins as it lacks a DRYLAIVHA cytoplasmic motif. DARC has three potential functions: first, it binds circulating inflammatory-type chemokines, serving as a repository for future chemokine release; second, it acts as a vehicle by which chemokines are transported from the abluminal to the luminal side of endothelium; and third, it complexes with signal-transducing chemokine receptors to create a nonsignaling heterodimer. Mouse DARC is 334 amino acids (aa) in length. It contains a 62 aa N-terminal extracellular region, and a 28 aa C-terminal cytoplasmic tail. There is one potential splice variant that shows a 42 aa substitution for aa 133-334. Collectively, over the four extracellular domains (aa 1-62, 115-127, 186-205, 264-285), mouse DARC shares 52% and 75% aa identity with human and rat DARC, respectively.

Long Name
Duffy Antigen, Receptor For Chemokines
Entrez Gene IDs
2532 (Human); 13349 (Mouse)
Alternate Names
ACKR1; CCBP1; CD234 antigen; CD234; DARC; Dfy; Duffy Antigen; Duffy antigen/chemokine receptor; Duffy blood group antigen; Duffy blood group, chemokine receptor; Fy Antigen; Fy glycoprotein; FYDuffy blood group; Glycoprotein D; GPD; GPDWBCQ1; GpFy; Plasmodium vivax receptor

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Citation for Mouse/Rat DARC APC-conjugated Antibody

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1 Citation: Showing 1 - 1

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