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Mouse/Rat CCR9 APC-conjugated Antibody

Catalog #: FAB2160A
2 Citations 1 Review Datasheet / COA / SDS

Discontinued Product

FAB2160A has been discontinued.
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Detection of CCR9 in Rat Splenocytes by Flow Cytometry.
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Product Details
Citations (2)
FAQs
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat CCR9 APC-conjugated Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat CCR9 in flow cytometry. Stains mouse CCR9-transfected cells but not irrelevant transfectants.
Source
Monoclonal Rat IgG2B Clone # 242503
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with mouse CCR9
Met1-Leu369
Accession # Q9WUT7
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR9 antibody in Rat Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR9 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Rat Anti-Mouse/Rat CCR9 APC-conjugated Monoclonal Antibody (Catalog # FAB2160A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR9 antibody in Mouse Thymocytes antibody by Flow Cytometry. View Larger

Detection of CCR9 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse/Rat CCR9 APC-conjugated Monoclonal Antibody (Catalog # FAB2160A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR9

CCR9 is a G protein-linked seven transmembrane domain chemokine receptor that serves as a receptor for CCL25/TECK. CCR9 is expressed on mature and immature thymocytes and some peripheral T and B cells.

Entrez Gene IDs
10803 (Human); 12769 (Mouse); 282832 (Rat)
Alternate Names
C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR9; CCR-9; CD199; CDw199 antigen; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6G-protein coupled receptor 28

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Citations for Mouse/Rat CCR9 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Regulation of humoral and cellular gut immunity by lamina propria dendritic cells expressing Toll-like receptor 5.
    Authors: Uematsu S, Fujimoto K, Jang MH, Yang BG, Jung YJ, Nishiyama M, Sato S, Tsujimura T, Yamamoto M, Yokota Y, Kiyono H, Miyasaka M, Ishii KJ, Akira S
    Nat. Immunol., 2008-05-30;9(7):769-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Sphingosine 1-phosphate regulates the egress of IgA plasmablasts from Peyer's patches for intestinal IgA responses.
    Authors: Gohda M, Kunisawa J, Miura F, Kagiyama Y, Kurashima Y, Higuchi M, Ishikawa I, Ogahara I, Kiyono H
    J. Immunol., 2008-04-15;180(8):5335-43.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Reviews for Mouse/Rat CCR9 APC-conjugated Antibody

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Mouse/Rat CCR9 APC‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: activated mouse CD8 T cell Species: Mouse

Cells were stained for 10 minutes in room temperature followed by 3 washes.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS + 10% FBS
Dilution: 1/10

Flow Cytometry Mouse/Rat CCR9 APC‑conjugated Antibody FAB2160A