Mouse OX40/TNFRSF4 Biotinylated Antibody

Catalog #: BAF1256 Datasheet / COA / SDS

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BAF1256

All OX40 Antibodies

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Mouse OX40/TNFRSF4 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse OX40/TNFRSF4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) 4-1BB, rmCD27, rmCD30, rmCD40, rmDR3, rmEDAR, rmFas, rmGITR, rmOPG, rmRANK, rmTNF RI, and rmTNF RII is observed.

Source

Polyclonal Goat IgG

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse OX40/TNFRSF4 Fc Chimera (Catalog # 1256-OX)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: OX40

OX40, also known as CD134, was originally identified as an activated rat CD4⁺ T cell-surface antigen that is recognized by the monoclonal antibody MRC OX40. It is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF4. Mouse OX40 cDNA encodes a 256 amino acid (aa) residues type I transmembrane precursor protein with a putative 19 aa signal peptide, a 192 aa extracellular domain containing 4 TNFR-cysteine rich repeats, a 25 aa transmembrane domain and a 36 aa cytoplasmic region. A naturally occurring soluble OX40 has also been identified in human serum. Mouse OX40 shares approximately 63% and 90% aa sequence identity with its human and rat counterparts, respectively. OX40 is a T cell activation antigen that is expressed primarily on activated CD4⁺ T cells, but is also expressed on activated human and mouse CD8⁺T cells. The ligand of OX40 is OX40 ligand (OX40L), also known as gp34, a type II transmembrane glycoprotein belonging to the TNF superfamily. OX40L is expressed on activated B cells, T cells, dendritic cells and endothelial cells. Ligation of OX40 on T cells by OX40L or an agonistic antibody can promote clonal expansion, long-term T cell survival, and enhance memory T cell development. In vivo, blockade of OX40/OX40L interaction has been useful for treating autoimmune disease and graft-versus-host disease in animal models. Activation of OX40 has also been utilized to enhance the potency of vaccines and augment anti-tumor immunity (1-9).

References

Paterson, D.J. et al. (1987) Mol. Immunol. 24:1281.
Mallett, S. et al. (1990) EMBO J. 9:1063.
Calderhead, D.M. et al. (1993) J. Immunol. 151:5261.
Baum, P.R. et al. (1994) EMBO J. 13:3992.
Gramaglia, I. et al. (1998) J. Immunol. 161:6510.
Bansal-Pakala, P. et al. (2001) Nature Medicine 7:907.
Rogers, P.R. et al. (2001) Immunity 15:445.
Taylor, L. and H.J. Schwarz (2001) Immunol. Methods 255:67.
Weinberg, A.D. (2002) Trends in Immunol. 23:102.

Entrez Gene IDs

7293 (Human); 22163 (Mouse); 25572 (Rat); 102145978 (Cynomolgus Monkey)

Alternate Names

ACT-135; ACT35 antigen; ACT35ATC35 antigen; CD134 antigen; CD134; Ly-70; OX40 cell surface antigen; OX40 homologue; OX40; OX40L receptor; OX40lymphoid activation antigene ACT35; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1 receptor; TNFRSF4; tumor necrosis factor receptor superfamily member 4; tumor necrosis factor receptor superfamily, member 4; Txgp1; TXGP1L; TXGP1LOX40 antigen