Mouse NKG2A/CD159a Antibody

Catalog #: MAB68671 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB68671-100
MAB68671-SP

Detection of NKG2A/CD159a in HEK293 Human Cell Line Transfected with Mouse NKG2A/CD159a and eGFP by Flow Cytometry.

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All NKG2A/CD159a Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse NKG2A/CD159a Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse NKG2A/CD159a in direct ELISAs. In Flow Cytometry, no cross-reactivity with mouse NKG2C/CD159c is observed.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2097C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Synthtic peptide containing mouse NKG2A/CD159a
Accession # Q9Z202

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NKG2A/CD159a in HEK293 Human Cell Line Transfected with Mouse NKG2A/CD159a and eGFP by Flow Cytometry. HEK293 Human embryonic kidney cell line transfected with either (A) Mouse NKG2A/CD159a, or (B) Mouse NKG2C/CD159c and eGFP was stained with Rabbit Anti-Mouse NKG2A/CD159a Monoclonal Antibody (Catalog # MAB68671) followed by APC-conjugated anti-Rabbit IgG secondary antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2A/CD159a

NKG2A, also known as CD159a, is a 244 amino acid (aa), ~38 kDa type II transmembrane glycoprotein with a cytoplasmic ITIM motif and a single extracellular lectin-like domain. Within the extracellular domain (aa 94-244), mouse NKG2A shares 71% and 41% aa sequence identity with rat and human NKG2A, respectively. An isoform lacking aa 98-114 has been described. NKG2A associates with CD94 and is coexpressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its murine ligand, Qa-1b (or HLA-E in humans) on antigen presenting cells.

Long Name

Natural Killer G2A

Entrez Gene IDs

3821 (Human); 16641 (Mouse); 102121106 (Cynomolgus Monkey)

Alternate Names

CD159 antigen-like family member A; CD159a antigen; CD159a; C-lectin type II protein; killer cell lectin-like receptor subfamily C, member 1; Klrc1; MGC13374; natural killer cell lectin; natural killer group protein 2; NK cell receptor A; NKG2; NKG2-1/B activating NK receptor; NKG2A; NKG2-A; NKG2-A/B type II integral membrane protein; NKG2-A/B-activating NK receptor; NKG2-A/NKG2-B type II integral membrane protein; NKG2AMGC59791; NKG2-B