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Mouse NGFR/TNFRSF16 Antibody

Catalog #: AF1157
25 Citations 6 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1157
AF1157-SP
Detection of Mouse NGF R/TNFRSF16 by Western Blot.
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Product Details
Citations (25)
FAQs
Supplemental Products
Reviews (6)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse NGFR/TNFRSF16 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse NGF R/TNFRSF16 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human NGF R/TNFRSF16 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NGF R/TNFRSF16
Gly20-Asn243
Accession # Q9Z0W1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse NGF R/TNFRSF16 antibody by Western Blot. View Larger

Detection of Mouse NGF R/TNFRSF16 by Western Blot. Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1157) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for NGF R/TNFRSF16 at approximately 60-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry NGF R/TNFRSF16 antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

NGF R/TNFRSF16 in Mouse Brain. NGF R/TNFRSF16 was detected in perfusion fixed frozen sections of mouse brain (cortex) using 7 µg/mL Goat Anti-Mouse NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1157) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NGFR/TNFRSF16

The low affinity nerve growth factor receptor (NGF R), also named p75 neurotrophin receptor, is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family and has been designated TNFRSF16. NGF R cDNA encodes a 427 amino acid (aa) residue precursor protein with a 28 aa residue signal peptide, a 222 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 155 aa residue intracellular domain. The extracellular region contains four cysteine-rich domains and binds NGF, BDNF, NT-3, and NT-4 approximately equally with low affinity. The cytoplasmic region of the receptor contains a subtype 2 death domain.

NGF R expression has been shown to occur widely during development and in the adult. Expression has been detected in both neuronal and non-neuronal cells. NGF R was originally reported to function as a positive regulator of TrkA activity. NGF R has also been shown to signal by itself. Depending on its cellular environment, NGF R has now been shown to regulate cell migration, gene expression and to mediate apoptosis. Recombinant NGF R Fc chimera binds NGF with high affinity and is a potent NGF antagonist. Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma, and in the amniotic fluid of humans and rats (1-3).

References
  1. Barker, P.A. and R.A. Murphy (1992) Molecular and Cellular Biochemistry 110:1.
  2. Bamji, A.X. et al. (1998) J. Cell Biol. 140:911.
  3. Feinstein, E. et al. (1995) Trends Biochem. Sci. 20:342.
Long Name
Nerve Growth Factor Receptor
Entrez Gene IDs
4804 (Human); 18053 (Mouse); 117089 (Rat)
Alternate Names
CD271 antigen; CD271; Gp80-LNGFR; member 16; nerve growth factor receptor; NGF R; NGF receptor; NGFR; p75 ICD; p75 NTR; TNFRSF16; TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16); tumor necrosis factor receptor superfamily member 16

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Citations for Mouse NGFR/TNFRSF16 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Regulation of BACE1 expression after injury is linked to the p75 neurotrophin receptor
    Authors: Khalil Saadipour, Alexia Tiberi, Sylvia Lombardo, Elena Grajales, Laura Montroull, Noralyn B. Mañucat-Tan et al.
    Molecular and Cellular Neuroscience
  2. Blockade of TrkB but not p75 NTR activates a subpopulation of quiescent neural precursor cells and enhances neurogenesis in the adult mouse hippocampus
    Authors: Natalie Groves, Imogen O’Keeffe, Wendy Lee, Alexandra Toft, Daniel Blackmore, Saurabh Bandhavkar et al.
    Developmental Neurobiology
  3. Cortical Brain Injury Causes Retrograde Degeneration of Afferent Basal Forebrain Cholinergic Neurons via the p75NTR
    Authors: Srestha Dasgupta, Laura E. Montroull, Mansi A. Pandya, Juan P. Zanin, Wei Wang, Zhuhao Wu et al.
    eNeuro
  4. TrkB-containing exosomes promote the transfer of glioblastoma aggressiveness to YKL-40-inactivated glioblastoma cells
    Authors: Sandra Pinet, Barbara Bessette, Nicolas Vedrenne, Aurélie Lacroix, Laurence Richard, Marie-Odile Jauberteau et al.
    Oncotarget
  5. Neurotrophic factor control of adult SVZ neurogenesis
    Authors: Kevin G. Bath, Francis S. Lee
    Developmental Neurobiology
  6. Neuronal apoptosis induced by morphine withdrawal is mediated by the p75 neurotrophin receptor
    Authors: Gino P. Asuni, Andrew Speidell, Italo Mocchetti
    Journal of Neurochemistry
  7. Proneurotrophins Induce Apoptotic Neuronal Death After Controlled Cortical Impact Injury in Adult Mice
    Authors: Laura E. Montroull, Deborah E. Rothbard, Hur D. Kanal, Veera D'Mello, Vincent Dodson, Carol M. Troy et al.
    ASN Neuro
  8. p75 Neurotrophin Receptor Activation Regulates the Timing of the Maturation of Cortical Parvalbumin Interneuron Connectivity and Promotes Juvenile-like Plasticity in Adult Visual Cortex
    Authors: Elie Baho, Bidisha Chattopadhyaya, Marisol Lavertu-Jolin, Raffaele Mazziotti, Patricia N. Awad, Pegah Chehrazi et al.
    The Journal of Neuroscience
  9. The p75 neurotrophin receptor is required for the survival of neuronal progenitors and normal formation of the basal forebrain, striatum, thalamus and neocortex
    Authors: Sonja Meier, Fabienne Alfonsi, Nyoman D. Kurniawan, Michael R. Milne, Maria A. Kasherman, Alessio Delogu et al.
    Development
  10. Tiered sympathetic control of cardiac function revealed by viral tracing and single cell transcriptome profiling
    Authors: Sharma S, Littman R, Tompkins JD et al.
    eLife
  11. Neuroimaging, Urinary, and Plasma Biomarkers of Treatment Response in Huntington’s Disease: Preclinical Evidence with the p75NTR Ligand LM11A-31
    Authors: Danielle A. Simmons, Brian D. Mills, Robert R. Butler III, Jason Kuan, Tyne L. M. McHugh, Carolyn Akers et al.
    Neurotherapeutics
  12. Tiered Sympathetic Control of Cardiac Function Revealed by Viral Tracing and Single Cell Transcriptome Profiling
    Authors: S Sharma, R Littman, J Tompkins, D Arneson, J Contreras, AH Dajani, K Ang, A Tsanhani, X Sun, PY Jay, H Herzog, X Yang, OA Ajijola
    bioRxiv : the preprint server for biology, 2023-01-19;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Closing the Gap between the Auditory Nerve and Cochlear Implant Electrodes: Which Neurotrophin Cocktail Performs Best for Axonal Outgrowth and Is Electrical Stimulation Beneficial?
    Authors: D Schmidbaue, S Fink, F Rousset, H Löwenheim, P Senn, R Glueckert
    International Journal of Molecular Sciences, 2023-01-19;24(3):.
    Species: Mouse
    Sample Types: Organoid
    Applications: IHC
  14. Etiological Roles of p75NTR in a Mouse Model of Wet Age-Related Macular Degeneration
    Authors: PV Subirada, A Tovo, MV Vaglienti, JD Luna Pinto, HU Saragovi, MC Sánchez, A Anastasía, PF Barcelona
    Cells, 2023-01-12;12(2):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Sterol O-Acyltransferase Inhibition Ameliorates High-Fat Diet-Induced Renal Fibrosis and Tertiary Lymphoid Tissue Maturation after Ischemic Reperfusion Injury
    Authors: Y Ariyasu, Y Sato, Y Isobe, K Taniguchi, M Yanagita, M Arita
    International Journal of Molecular Sciences, 2022-12-07;23(24):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. A Novel Neuroprotective Mechanism for Lithium That Prevents Association of the p75NTR-Sortilin Receptor Complex and Attenuates proNGF-Induced Neuronal Death In Vitro and In Vivo
    Authors: SG Greenwood, L Montroull, M Volosin, HE Scharfman, KK Teng, M Light, R Torkin, F Maxfield, BL Hempstead, WJ Friedman
    eNeuro, 2018-01-17;5(1):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  17. Urinary Extracellular Domain of Neurotrophin Receptor p75 as a Biomarker for Amyotrophic Lateral Sclerosis in a Chinese cohort
    Authors: R Jia, S Shepheard, J Jin, F Hu, X Zhao, L Xue, L Xiang, H Qi, Q Qu, F Guo, ML Rogers, J Dang
    Sci Rep, 2017-07-11;7(1):5127.
    Species: Human
    Sample Types: Urine
    Applications: ELISA Development (Detection)
  18. Superior Cervical Ganglia Neurons Induce Foxp3+ Regulatory T Cells via Calcitonin Gene-Related Peptide
    Authors: K Szklany, E Ruiter, F Mian, W Kunze, J Bienenstoc, P Forsythe, K Karimi
    PLoS ONE, 2016-03-29;11(3):e0152443.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Semaphorin 3E secreted by damaged hepatocytes regulates the sinusoidal regeneration and liver fibrosis during liver regeneration.
    Authors: Yagai T, Miyajima A, Tanaka M
    Am J Pathol, 2014-06-12;184(8):2250-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function.
    Authors: Matusica, Dusan, Skeldal, Sune, Sykes, Alex M, Palstra, Nickless, Sharma, Aanchal, Coulson, Elizabet
    J Biol Chem, 2013-03-07;288(16):11144-54.
    Species: Human, Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Mouse natural killer (NK) cells express the nerve growth factor receptor TrkA, which is dynamically regulated.
    Authors: Ralainirina N, Brons NH, Ammerlaan W, Hoffmann C, Hentges F, Zimmer J
    PLoS ONE, 2010-12-01;5(12):e15053.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  22. Long-term delivery of nerve growth factor by encapsulated cell biodelivery in the Gottingen minipig basal forebrain.
    Authors: Fjord-Larsen L, Kusk P, Tornoe J, Juliusson B, Torp M, Bjarkam CR, Nielsen MS, Handberg A, Sorensen JC, Wahlberg LU
    Mol. Ther., 2010-07-27;18(12):2164-72.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  23. Human immunomodulatory ligand B7-1 mediates synaptic remodeling via the p75 neurotrophin receptor
    Authors: Nicholas C. Morano, Roshelle S. Smith, Victor Danelon, Ryan Schreiner, Uttsav Patel, Natalia G. Herrera et al.
    Journal of Clinical Investigation
  24. Heterogeneous fibroblasts underlie age-dependent tertiary lymphoid tissues in the kidney
    Authors: Yuki Sato, Akiko Mii, Yoko Hamazaki, Harumi Fujita, Hirosuke Nakata, Kyoko Masuda et al.
    JCI Insight
  25. BMSCs Promote Differentiation of Enteric Neural Precursor Cells to Maintain Neuronal Homeostasis in Mice With Enteric Nerve Injury
    Authors: Mengke Fan, Huiying Shi, Hailing Yao, Weijun Wang, Yurui Zhang, Chen Jiang et al.
    Cellular and Molecular Gastroenterology and Hepatology

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Reviews for Mouse NGFR/TNFRSF16 Antibody

Average Rating: 4.7 (Based on 6 Reviews)

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Mouse NGFR/TNFRSF16 Antibody
By Valeria Avdoshina on 11/18/2019
Application: WB Sample Tested: Brain (hippocampus) tissue Species: Mouse

2 microgram per mL Primary antibody dilution.

Western Blot Mouse NGFR/TNFRSF16 Antibody AF1157
Mouse NGFR/TNFRSF16 Antibody
By Valeria Avdoshina on 11/18/2019
Application: IHC Sample Tested: Brain (forebrain) Species: Mouse

Primary antibody dilution: 5 microgram per mL

Immunohistochemistry Mouse NGFR/TNFRSF16 Antibody AF1157
Mouse NGF R/TNFRSF16 Antibody
By Anonymous on 11/02/2017
Application: IHC Sample Tested: Cerebellum tissue Species: Mouse

Postnatal day 7 mouse cerebellar tissue.

Immunohistochemistry Mouse NGF R/TNFRSF16 Antibody AF1157
Mouse NGF R/TNFRSF16 Antibody
By Anonymous on 10/17/2016
Application: IHC Sample Tested: Brain (cortex) tissue Species: Mouse

PN18 mouse
Closed Head Injury
Dilution 1:500

Immunohistochemistry Mouse NGF R/TNFRSF16 Antibody AF1157
Mouse NGF R/TNFRSF16 Antibody
By Anonymous on 07/26/2016
Application: IHC Sample Tested: Brain (cerebral cortex) Species: Mouse

Frozen section. 20um thickness. Antibody dilution 1/500.

Immunohistochemistry Mouse NGF R/TNFRSF16 Antibody AF1157
Mouse NGF R/TNFRSF16 Antibody
By Juan Zanin on 07/13/2016
Application: IHC Sample Tested: Cerebellum tissue Species: Mouse and Rat

Cryostat Frozen section. 20um ticknes. Antibody dilution 1/500 ON 4C.

Immunohistochemistry Mouse NGF R/TNFRSF16 Antibody AF1157