Mouse Neuropilin-1 PE-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Neuropilin-1

Neuropilin-1 (Nrp-1, previously neuropilin; also CD304) is a 130 - 140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis. The full-length 923 amino acid (aa) mouse Nrp-1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with rat and 93% aa identity with human, equine, bovine and canine Nrp-1. The ECD contains two N-terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). At least one splice variant that diverges at aa 587 and lacks the TM domain has been sequenced. This form is potentially a soluble antagonist, based on results from human Nrp-1 splice variants. The sema domains of Class III secreted semaphorins such as Sema3A bind Nrp-1 a1a2. Heparin, the heparin-binding forms of VEGF (VEGF₁₆₅, VEGF-B and VEGF-E), PlGF (PlGF2), plus the C-terminus of Sema3 bind the b1b2 region of Nrp-1. Nrp-1 and Nrp-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains. Neuropilins show partially overlapping expression in neuronal and endothelial cells during development. Both neuropilins act as co-receptors with plexins, including plexins A1, A4, B1, and D1, to bind class III semaphorins, such as Sema 3A, 3C, 3D, 3E, and 3F, that often mediate axon repulsion. Both Nrp-1 and Nrp-2 serve as co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF₁₆₅ binding. Sema3A signaling can be blocked by VEGF₁₆₅, which has higher affinity for Npn-1. Nrp-1 is preferentially expressed in developing or remodeling arteries. Nrp-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation.

Product Datasheets

Related Research Areas

• Cytokine and Growth Factor Receptors on VSMC
• Neuronal Markers
• Plasmacytoid Dendritic Cells
• Regulatory T Cells (Tregs)
• Semaphorins, Plexin Receptors, and Related Molecules
• VEGF Family
• VEGF Receptors and Regulators on Endothelial Cells