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Mouse MFG-E8 Antibody

Catalog #: MAB2805
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2805-SP
MAB2805-100
MAB2805-500
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MFG-E8 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MFG-E8 in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 340614
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MFG-E8
Ala23-Cys463
Accession # P21956
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse MFG-E8 (Catalog # 2805-MF)
under non-reducing conditions only

Mouse MFG-E8 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse MFG‑E8 Biotinylated Antibody (Catalog # BAF2805)

Standard: Recombinant Mouse MFG-E8 Protein (Catalog # 2805-MF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MFG-E8

Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66‑75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Mouse MGF-E8 contains two N-terminal EGF-like domains, a Pro/Thr-rich segment, and two C-terminal F5/8-type discoidin-like domains (2). It MFG-E8 shares 63% and 94% aa sequence identity with comparable regions of human and rat MFG-E8, respectively. Alternative splicing of mouse MFG-E8 generates a short isoform lacking the Pro/Thr-rich region which contains sites for O-linked glycosylation and tyrosine sulfation (3). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (4, 5). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (6). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (7, 8) and of apoptotic B cells during germinal center reactions (9, 10). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (11, 12). Its tissue-protective role impairs anti‑tumor immunity and chemotherapy-induced apoptosis (13). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (14).

References
  1. Raymond, A. et al. (2009) J. Cell. Biochem. 106:957.
  2. Stubbs, J.D. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8417.
  3. Hoffhines, A.J. et al. (2008) J. Biol. Chem. 284:3096.
  4. Silvestre, J.-S. et al. (2005) Nat. Med. 11:499.
  5. Borges, E. et al. (2000) J. Biol. Chem. 275:39867.
  6. Hanayama, R. et al. (2002) Nature 417:182.
  7. Ait-Oufella, H. et al. (2007) Circulation 115:2168.
  8. Kranich, J. et al. (2010) J. Exp. Med. 207:2271.
  9. Hanayama, R. et al. (2004) Science 304:1147.
  10. Kranich, J. et al. (2010) J. Exp. Med. 205:1293.
  11. Atabai, K. et al. (2009) J. Clin. Invest. 119:3713.
  12. Bu, H.-F. et al. (2007) J. Clin. Invest. 117:3673.
  13. Jinushi, M. et al. (2009) J. Exp. Med. 206:1317.
  14. Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.
Long Name
Milk Fat Globule EGF Factor 8
Entrez Gene IDs
4240 (Human); 17304 (Mouse)
Alternate Names
BA46; Breast epithelial antigen BA46; EDIL1; hP47; HsT19888; lactadherin; Lactahedrin; Medin; MFG1; MFGE8; MFG-E8; MFGM; milk fat globule-EGF factor 8 protein; Milk fat globule-EGF factor 8; O-acetyl disialoganglioside synthase; OAcGD3S; SED1; SPAG10; sperm associated antigen 10; sperm surface protein hP47

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Citations for Mouse MFG-E8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Living Neurons with Tau Filaments Aberrantly Expose Phosphatidylserine and Are Phagocytosed by Microglia
    Authors: J Brelstaff, AM Tolkovsky, B Ghetti, M Goedert, MG Spillantin
    Cell Rep, 2018-08-21;24(8):1939-1948.e4.
  2. Propofol suppresses microglial phagocytosis through the downregulation of MFG-E8
    Authors: X Cai, Y Li, X Zheng, R Hu, Y Li, L Xiao, Z Wang
    Journal of Neuroinflammation, 2021-01-09;18(1):18.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  3. Aging-Associated Alterations in Mammary Epithelia and Stroma Revealed by Single-Cell RNA Sequencing
    Authors: CM Li, H Shapiro, C Tsiobikas, LM Selfors, H Chen, J Rosenbluth, K Moore, KP Gupta, GK Gray, Y Oren, MJ Steinbaugh, JL Guerriero, L Pinello, A Regev, JS Brugge
    Cell Reports, 2020-12-29;33(13):108566.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. An integrin/MFG-E8 shuttle loads HIV-1 viral-like particles onto follicular dendritic cells in mouse lymph node
    Authors: Chung Park, John H Kehrl
    eLife

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