Mouse Marapsin/Pancreasin Antibody

Catalog #: AF1989 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1989
AF1989-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Marapsin/Pancreasin Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Marapsin/Pancreasin in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Marapsin is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Marapsin/Pancreasin
Ala23-Thr290
Accession # Q8BJR6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Marapsin/Pancreasin (Catalog # 1989-SE)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse Marapsin/Pancreasin (Catalog # 1989-SE), see our available Western blot detection antibodies

Neutralization

Measured by its ability to neutralize Recombinant Mouse Marapsin/Pancreasin (0.5 µg/mL, Catalog # 1989-SE) cleavage of the colorimetric peptide substrate Z-GR-SBzl (100 µM) in the presence of DTNB (100 µM). The Neutralization Dose (ND₅₀) is typically 5 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Marapsin/Pancreasin

Marapsin, Pancreasin, and channel-activating protease 2 (CAP-2), encoded by the Prss27 gene, are different names given for the same serine protease that is expressed strongly in the pancreas (1). The mouse protein is synthesized with a signal peptide (amino acid residues 1‑22), a pro peptide (residues 23‑37) and a mature chain (residues 38‑290) corresponding to the serine protease domain. The full-length protein was expressed and the secreted protein purified. The N-terminal sequencing results indicate that the purified protein is a disulfide bond-linked dimer formed between the pro peptide and the mature chain. The active enzyme has low activity against peptide substrates tested, but high activity against thioester substrates. The peptidase activity is inhibited by 20 mM benzamidine.

Entrez Gene IDs

83886 (Human); 213171 (Mouse)

Alternate Names

CAP-2; CAPH2; channel-activating protease 2; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; EC 3.4.21.43; Marapsin; MPN; MPNMarapsin; Pancreasin; protease, serine 27; PRSS27; serine protease 27