Mouse LRIG1 Antibody

Detection of Mouse LRIG1 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line untreated (-), treated (+) or mock treated (m) with 30 pmol mouse LRIG1 or mouse ERK1 siRNA. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for LRIG1 at approximately 140 kDa and 70 kDa (as indicated, upper panel). For additional reference ERK1 was detected using Rabbit Anti-Human/Mouse/Rat ERK1 Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF1575) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of LRIG1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

LRIG1 in bEnd.3 Mouse Cell Line. LRIG1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Mouse LRIG1 by Immunohistochemistry JunB expression during skin maturation and upon stress. a Representative microphotographs with double immunostaining for JunB (green) and FABP5 (red), indicative of sebaceous glands, in skin derived from 1, 3, and 5 days old mice. Inset showing magnified view of a sebaceous gland. Scale bars, 50 µm. b Immunostaining of JunB (green) and FABP5 (red) in 60 days old mice. Inset showing magnified view. c Representative microphotographs with double immunostaining of JunB (green) and FABP5 (red) in dorsal skin of 60 days old mice following hair plucking or d after topical TPA application, a potent inducer of proliferation. e Immunostaining of JunB (green) and LRIG1 or CD34 (red) in dorsal skin of 60 days old mice following hair plucking. f Immunostaining of JunB (green) and FABP5 (red) in adult human skin. E, epidermis; D, dermis; HF, hair follicle; SG, sebaceous gland; SD, sebaceous duct; HS, hair shaft. Asterisk indicates hair shaft autofluorescence. Dashed line indicates the epidermal-dermal junction. Scale bars, 50 µm Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30143626), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse LRIG1 by Immunohistochemistry Lrigs are co-expressed in the embryonic inner ear.(A) Diagram of the immature inner ear structure at E12.5 (left) and the mature structure at E16 (right), with schematic cross sections cut transverse to the ear at each age shown below. For the E16 cross section, the sensory epithelia are labeled red and the neurons green. In situs for Lrig1 (B) and X-gal staining for Lrig3-beta geo activity (C) show overlapping expression for Lrig1 and Lrig3 in the atrium (arrowhead) and the non-sensory domain of the cochlea. On the other hand, Lrig2-beta geo is active throughout the developing otic epithelium (D). c = cochlea, ed = endolymphatic duct, lp = lateral pouch, vp = vertical pouch. Scale bar = 50 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24086156), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse LRIG1 by Immunocytochemistry/Immunofluorescence Lrig1 and Lrig2-beta geo are co-expressed in non-sensory tissues and in the vestibular ganglion.Transverse sections through Lrig2+/− tissue at E12.5 (A–C), E16.5 (D–G), and P15 (H–K) were double labeled with combinations of antibodies to Lrig1, beta -galactosidase (to detect Lrig2-beta geo), Sox2, and neurofilament (NF). (A) At E12.5, Lrig1 was detected in the atrium (bracket), while Lrig2-beta geo was present throughout the otic epithelium and surrounding mesenchyme (A″), overlapping with Lrig1 in the atrium (B). Within the atrium, Lrig1 was present in non-sensory tissues that flank Sox-2 positive sensory regions (arrowheads, C–C″). This expression was maintained at E16.5, with Lrig1 in the transitional epithelium adjacent to the utricular macula (arrowhead, D′) and in the extramacular epithelium of the saccule (arrowhead, F′), as well as in vestibular projections to the utricle and lateral crista (arrow, D′). Lrig2-beta geo, on the other hand, continued to be expressed broadly in both sensory and non-sensory portions of the vestibular organs at E16.5 (E′, G′). After the onset of hearing (P15), Lrig1 was expressed in NF-positive fibers innervating the utricular and saccular maculae (arrows, I, J), whereas Lrig2-beta geo was enriched in all vestibular sensory epithelia (I′,J′,K), which were recognized by the presence of NF labeled projections. c = crista, cd = cochlear duct, ed = endolymphatic duct, hb = hindbrain, lp = lateral pouch, sg = spiral ganglion, sm = saccular macula, um = utricular macule, vg = vestibular ganglion, vp = vertical pouch, VIIIV = vestibular division of the eighth cranial nerve. Scale bar = 40 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24086156), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse LRIG1 by Immunocytochemistry/Immunofluorescence Lrig1 and Lrig2-beta geo are co-expressed in the non-sensory region of the cochlea.Transverse sections through Lrig2+/− tissue at E12.5 (A), E16.5 (B), and P15 (C) were double labeled with antibodies to Lrig1, beta -galactosidase, and NF. (A) At E12.5, staining was evident in the non-sensory region of the cochlear epithelium (asterisk) and the mesenchyme surrounding the spiral ganglion. (B) At E16.5, Lrig1 was detected in the medial wall of the cochlea, which will form the inner sulcus and Reissner's membrane (asterisk). (C) At P15, Lrig1 was found in the base of Reissner's membrane (asterisk), with localization to the cell surface (inset). In contrast, at E12.5 and 16.5, Lrig2-beta geo was found broadly in the cochlear epithelium and surrounding mesenchyme (A′–B′). At P15, expression was enriched in spiral ganglion neurons and in the organ of Corti (C′). cd = cochlear duct, m = mesenchyme, oC = organ of Corti, rm = Reissner's membrane, sg = spiral ganglion. Scale bar = 40 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24086156), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse LRIG1 by Immunocytochemistry/Immunofluorescence Association of Oct1 with normal somatic stem cells.A. IF images of cross-sections from grossly uninvolved colon margins of a male familial adenomatous polyposis patient. Frozen sections were stained with mouse anti-Oct1 antibodies (Millipore MAB5434) and co-stained with TO-PRO. Crypts are shown in cross-section. White dashed circle highlights a crypt. Arrows indicate cells staining strongly for Oct1. B. IF images of colon crypt sections from a normal male individual. Sections were stained with DAPI, and anti-Oct1 and anti-ALDH1a1 antibodies. Merged images are shown at right. White dashed lines highlight crypts. Examples of cells co-staining with Oct1 and ALDH1a1 are highlighted with yellow arrows. An example cell staining with ALDH1a1 only is highlighted with an asterisk. Inset at lower right-hand corner is a digital magnification of the central portion of the image. Sections were formalin-fixed and paraffin-embedded. C. Frozen mouse colon tissue sections were stained with DAPI, and anti-Lrig1 and anti-Oct1 antibodies. IF images of longitudinal sections are shown. White dashed lines highlight the crypt. D. IF images of mouse small intestine sections. Sections were stained with DAPI and anti-Oct1 antibodies. Merged images are shown at right. White dashed lines highlight a crypt. Inset at upper right-hand corner is a digital magnification of the central portion of the image. Sections were formalin-fixed and paraffin-embedded. E. IF images of cross-sectional duodenum sections from a normal C57BL/6 mouse. Frozen sections were stained with DAPI and anti-Oct1 and anti-Lgr5 antibodies. Merged images are shown at right. Examples of co-staining cells are highlighted with yellow arrows. White dashed lines highlights crypts. F. Longitudinal sections. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23144633), licensed under a CC-BY license. Not internally tested by R&D Systems.