Mouse LIGHT/TNFSF14 Antibody

Name: Mouse LIGHT/TNFSF14 Antibody
Brand: R&D Systems
SKU: MAB1794
Rating: 5 (1 reviews)

Catalog #: MAB1794
2 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1794
MAB1794-SP

All LIGHT/TNFSF14 Antibodies

Product Details

Citations (2)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse LIGHT/TNFSF14 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse LIGHT in direct ELISAs and Western blots. In Western blots, this antibody shows less than 10% cross-reactivity with recombinant mouse (rm) EDA, rmTRAIL, rmTRANCE, rmTWEAK, and rhVEGI but does not cross-react rm4-1BB Ligand, rhAPRIL, rmBAFF, rhEDA-A2, rmFas Ligand, rhGITR Ligand, rmGITR Ligand, rhLymphotoxin alpha ₁/ beta ₂, rhLymphotoxin alpha ₂/ beta ₂, rmOX40 Ligand, and rmTNF-alpha.

Source

Monoclonal Rat IgG_2A Clone # 261639

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse LIGHT
Asp72-Val239
Accession # Q9QYH9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse LIGHT/TNFSF14 (Catalog # 1794-LT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIGHT/TNFSF14

LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) is a member of the TNF superfamily and is designated TNFSF14. The gene for mouse LIGHT encodes a 239 amino acid residue (aa) type II transmembrane glycoprotein that contains a 37 aa N-terminal cytoplasmic domain, a 21 aa transmembrane region, and a 181 aa extracellular domain. A soluble form of mouse LIGHT is generated from the membrane form by proteolytic processing. Similar to other TNF ligand family members, LIGHT is assembled as a homotrimer. Mouse and human LIGHT share 71% aa sequence identity. LIGHT is expressed by activated lymphocytes, natural killer cells, immature dendritic cells, monocytes and granulocytes. Mouse LIGHT binds and signals via two distinct TNF receptor superfamily members, including the herpes virus entry mediator (HVEM/TNFRSF14) and the lymphotoxin beta receptor (LT beta R/TNFRSF3). In humans, LIGHT also binds the soluble human decoy receptor 3 (DcR3/TNFRSF6B). Signaling from LT beta R, which also binds LT alpha beta, induces apoptosis and the production of various cytokines. LIGHT-LT beta R signaling also plays a role in mesenteric lymph node organogenesis, and restoration of secondary lymphoid structure and function. Signaling from HVEM, which also binds LT alpha, co-stimulates T-helper cell type 1 (TH1) immune responses, enhances Cytotoxic T Lymphocytes (CTL)-mediated tumor immunity, and regulates allogeneic T cell activation and allograft rejection. Blockade of LIGHT-HVEM signaling has been shown to prevent graft versus host disease.

References

Granger, S.W. and S. Rickert (2003) Cytokine Growth Factor Rev. 14:289.

Long Name

TNF Ligand Superfamily Member 14

Entrez Gene IDs

8740 (Human); 50930 (Mouse)

Alternate Names

CD258 antigen; CD258; delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator ligand; HVEM-L; HVEMLherpesvirus entry mediator-ligand; ligand for herpesvirus entry mediator; LIGHT; LIGHTherpesvirus entry mediator A; LTg; TNFSF14; TR2; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand superfamily member 14; tumor necrosis factor receptor-like 2; tumor necrosis factor superfamily member LIGHT

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse LIGHT/TNFSF14 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Anti-CD19 CAR T cells potently redirected to kill solid tumor cells
Authors: C Ambrose, L Su, L Wu, FJ Dufort, T Sanford, A Birt, BJ Hackel, A Hombach, H Abken, RR Lobb, PD Rennert
PLoS ONE, 2021-03-18;16(3):e0247701.
Proinflammatory clearance of apoptotic neutrophils induces an IL-12(low)IL-10(high) regulatory phenotype in macrophages.
Authors: Filardy AA, Pires DR, Nunes MP, Takiya CM, Freire-de-Lima CG, Ribeiro-Gomes FL, DosReis GA
J. Immunol., 2010-07-21;185(4):2044-50.
Species: Mouse
Sample Types: Whole Cells
Applications: ELISA Development