Mouse Integrin  alpha 9 Antibody

Catalog # Availability Size / Price Qty
AF3827
AF3827-SP
Integrin alpha 9 in Mouse Liver Tissue.
3 Images
Product Details
Citations (31)
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Mouse Integrin  alpha 9 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha 9 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant human Integrin alpha 9 is observed and less than 5% cross-reactivity with recombinant mouse Integrin alpha 3 and alpha 4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Integin alpha 9
Try31-Val979
Accession # NP_598482
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Integrin  alpha 9
Flow Cytometry
2.5 µg/106 cells
D3 mouse embryonic stem cell line
Immunohistochemistry
1-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Integrin alpha 9 antibody in Mouse Liver Tissue by Immunohistochemistry (IHC-Fr). View Larger

Integrin alpha 9 in Mouse Liver Tissue. Integrin a9 was detected in perfusion fixed frozen sections of mouse liver tissue using Goat Anti-Mouse Integrin a9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3827) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to bile canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry/ Immunofluorescence Detection of Human Integrin alpha 9 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Integrin alpha 9 by Immunocytochemistry/Immunofluorescence Morphology and protein expression of hSKPs. (a) SEM micrographs of nanofibrous scaffolds showing morphology of hSKPs at 7 days of culture. (b) Immunofluorescent images of hSKPs grown on nanofibrous scaffolds showing expression of integrin alpha -9 (green), fibronectin (red) and procollagen I & III (green). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28860484), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Integrin alpha 9 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Integrin alpha 9 by Immunocytochemistry/Immunofluorescence Intergins alpha 4/ alpha 9 are responsible for the action of osteopontin (OPN) in the augmentation of macrophage (Mφ) M2 polarization and angiogenic capacity. (A) Relative mean fluorescence intensities (MFIs) of integrins alpha 4/ alpha 9 were measured by FACS analysis in each Mφ subset. Anti-integrin alpha 4 or alpha 9 antibodies (Abs) and each isotype-matched control Ab were used at 10 µg/mL, n = 4 [***p < 0.001 vs. untreated, ###p < 0.001 vs. interleukin (IL)-10 alone]. (B) Representative confocal laser scanning immunofluorescence overlay images of integrin alpha 4 (green) and DAPI (blue), as well as those of integrin alpha 9 (red) and DAPI (blue) in Mφ (–) and Mφ (IL-10 + IL-18). Scale bar represents 20 µm. (C) Relative MFI of CD163 was measured by FACS analysis in each Mφ subset. Anti-integrin alpha 4 or alpha 9 Abs and each isotype-matched control Ab were used at 10 µg/mL, n = 4 (***p < 0.001 vs. untreated, ###p < 0.001 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). (D) The total areas and lengths of tube-like structures were determined by the Matrigel tube formation assay where b.End5 was cocultured with each Mφ subset. Anti-integrin alpha 4 or alpha 9 Abs and each isotype-matched control Ab were used at 10 µg/mL, n = 6 (***p < 0.001, **p < 0.01, *p < 0.05 vs. untreated, ###p < 0.001, ##p < 0.01, #p < 0.05 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). Integrin alpha 4 = ITGA4; Integrin alpha 9 = ITGA9. All data are expressed as means ± SEM and were analyzed by a one-way ANOVA followed by Tukey’s test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29559970), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 9

Integrin alpha 9 is a 150 kDa type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes. It principally associates with the beta 1 integrin. alpha 9 beta 1 binds to VEGF-C, VEGF-D, and osteopontin. The 951 amino acid (aa) extracellular domain of mouse integrin alpha 9 contains three beta -propellor repeats and multiple PheGly-GlyAlaPro repeats. A potential proteolytic cleavage site in human alpha 9 ECD is absolutely conserved in mouse alpha 9 ECD (Arg566-Val567). The ECD of mouse integrin alpha 9 shares 95% and 89% aa sequence identity to rat and human integrin alpha 9, respectively.

Entrez Gene IDs
3680 (Human); 104099 (Mouse); 685004 (Rat)
Alternate Names
ALPHA-RLC; Integrin alpha 9; integrin alpha-9; Integrin alpha-RLC; integrin, alpha 9; ITGA4L; ITGA9; RLC; RLC-alpha

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Citations for Mouse Integrin  alpha 9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. VE-Cadherin Is Required for Lymphatic Valve Formation and Maintenance
    Authors: Yang Y, Cha B, Motawe ZY et al.
    Cell Rep
  2. Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion
    Authors: Shaohe Wang, Kazue Matsumoto, Samantha R. Lish, Alexander X. Cartagena-Rivera, Kenneth M. Yamada
    Cell
  3. Integrin ?9?1 deficiency does not impact the development of atherosclerosis in mice
    Authors: Jung, IH;Stitziel, NO;
    Heliyon
    Species: Mouse
    Sample Types: Tissue Lysates, Whole Tissue
    Applications: IHC/IF, Western Blot
  4. Organ function is preserved despite reorganization of niche architecture in the hair follicle
    Authors: Wei, H;Du, S;Parksong, J;Pasolli, HA;Matte-Martone, C;Regot, S;Gonzalez, LE;Xin, T;Greco, V;
    Cell stem cell
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Progenitor-derived endothelin controls dermal sheath contraction for hair follicle regression
    Authors: P Martino, R Sunkara, N Heitman, M Rangl, A Brown, N Saxena, L Grisanti, D Kohan, M Yanagisawa, M Rendl
    Nature Cell Biology, 2023-01-30;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Loss of Primary Cilia Protein IFT20 Dysregulates Lymphatic Vessel Patterning in Development and Inflammation
    Authors: Delayna Paulson, Rebecca Harms, Cody Ward, Mackenzie Latterell, Gregory J. Pazour, Darci M. Fink
    Frontiers in Cell and Developmental Biology
  7. Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing
    Authors: S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie
    Cell Stem Cell, 2020-08-04;27(3):396-412.e6.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Shear stimulation of FOXC1 and FOXC2 differentially regulates cytoskeletal activity during lymphatic valve maturation
    Authors: Pieter R Norden, Amélie Sabine, Ying Wang, Cansaran Saygili Demir, Ting Liu, Tatiana V Petrova et al.
    eLife
  9. GATA2 controls lymphatic endothelial cell junctional integrity and lymphovenous valve morphogenesis through miR-126
    Authors: Md. Riaj Mahamud, Xin Geng, Yen-Chun Ho, Boksik Cha, Yuenhee Kim, Jing Ma et al.
    Development
  10. Mechanically activated ion channel PIEZO1 is required for lymphatic valve formation
    Authors: K Nonomura, V Lukacs, DT Sweet, LM Goddard, A Kanie, T Whitwam, SS Ranade, T Fujimori, ML Kahn, A Patapoutia
    Proc. Natl. Acad. Sci. U.S.A., 2018-11-27;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis
    Authors: T Kobori, S Hamasaki, A Kitaura, Y Yamazaki, T Nishinaka, A Niwa, S Nakao, H Wake, S Mori, T Yoshino, M Nishibori, H Takahashi
    Front Immunol, 2018-03-06;9(0):334.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  12. Biocomposite nanofiber matrices to support ECM remodeling by human dermal progenitors and enhanced wound closure
    Authors: F Anjum, NA Agabalyan, HD Sparks, NL Rosin, MS Kallos, J Biernaskie
    Sci Rep, 2017-08-31;7(1):10291.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Defective lymphatic valve development and chylothorax in mice with a lymphatic-specific deletion of Connexin43
    Authors: Alexander M Simon
    Dev. Biol., 2016-11-27;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Integrin Alpha 9 Blockade Suppresses Lymphatic Valve Formation and Promotes Transplant Survival
    Invest. Ophthalmol. Vis. Sci., 2016-11-01;57(14):5935-5939.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development.
    Authors: Geng X, Cha B, Mahamud M, Lim K, Silasi-Mansat R, Uddin M, Miura N, Xia L, Simon A, Engel J, Chen H, Lupu F, Srinivasan R
    Dev Biol, 2015-11-02;409(1):218-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Kindlin-3-mediated integrin adhesion is dispensable for quiescent but essential for activated hematopoietic stem cells.
    Authors: Ruppert R, Moser M, Sperandio M, Rognoni E, Orban M, Liu W, Schulz A, Oostendorp R, Massberg S, Fassler R
    J Exp Med, 2015-08-17;212(9):1415-32.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Structural and functional features of central nervous system lymphatic vessels.
    Authors: Louveau A, Smirnov I, Keyes T, Eccles J, Rouhani S, Peske J, Derecki N, Castle D, Mandell J, Lee K, Harris T, Kipnis J
    Nature, 2015-06-01;523(7560):337-41.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Tie1 is required for lymphatic valve and collecting vessel development
    Authors: Xianghu Qu, Bin Zhou, H. Scott Scott Baldwin
    Developmental Biology
  19. The Schlemm's canal is a VEGF-C/VEGFR-3-responsive lymphatic-like vessel.
    Authors: Aspelund A, Tammela T, Antila S, Nurmi H, Leppanen V, Zarkada G, Stanczuk L, Francois M, Makinen T, Saharinen P, Immonen I, Alitalo K
    J Clin Invest, 2014-07-25;124(9):3975-86.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity.
    Authors: Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G
    J Clin Invest, 2014-07-25;124(9):3960-74.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Angiopoietin 2 regulates the transformation and integrity of lymphatic endothelial cell junctions.
    Authors: Zheng W, Nurmi H, Appak S, Sabine A, Bovay E, Korhonen E, Orsenigo F, Lohela M, D'Amico G, Holopainen T, Leow C, Dejana E, Petrova T, Augustin H, Alitalo K
    Genes Dev, 2014-07-15;28(14):1592-603.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  22. Corneal lymphatic valve formation in relation to lymphangiogenesis.
    Authors: Truong, Tan, Huang, Eric, Yuen, Don, Chen, Lu
    Invest Ophthalmol Vis Sci, 2014-03-25;55(3):1876-83.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Bone morphogenetic protein 9 (BMP9) controls lymphatic vessel maturation and valve formation
    Authors: Sandrine Levet, Delphine Ciais, Galina Merdzhanova, Christine Mallet, Teresa A. Zimmers, Se-Jin Lee et al.
    Blood
  24. Smooth muscle-endothelial cell communication activates Reelin signaling and regulates lymphatic vessel formation.
    J. Cell Biol., 2012-06-04;197(6):837-49.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Polydom/SVEP1 Is a Ligand for Integrin alpha9beta1.
    Authors: Sato-Nishiuchi R, Nakano I, Ozawa A, Sato Y, Takeichi M, Kiyozumi D, Yamazaki K, Yasunaga T, Futaki S, Sekiguchi K
    J. Biol. Chem., 2012-05-31;287(30):25615-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  26. Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration.
    Authors: Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T, Kubota Y
    Blood, 2012-05-02;119(23):5429-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Genes regulating lymphangiogenesis control venous valve formation and maintenance in mice.
    Authors: Bazigou E, Lyons OT, Smith A, Venn GE, Cope C, Brown NA, Makinen T
    J. Clin. Invest., 2011-07-18;121(8):2984-92.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  28. Short form of alpha 9 promotes alpha 9 beta 1 integrin-dependent cell adhesion by modulating the function of the full-length alpha 9 subunit
    Authors: Shigeyuki Kon, Amha Atakilit, Dean Sheppard
    Experimental Cell Research
  29. Single transcription factor reprogramming of hair follicle dermal papilla cells to induced pluripotent stem cells.
    Authors: Tsai SY, Bouwman BA, Ang YS, Kim SJ, Lee DF, Lemischka IR, Rendl M
    Stem Cells, 2011-06-01;29(6):964-71.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. Oct4 and klf4 reprogram dermal papilla cells into induced pluripotent stem cells.
    Authors: Tsai SY, Clavel C, Kim S, Ang YS, Grisanti L, Lee DF, Kelley K, Rendl M
    Stem Cells, 2010-02-01;28(2):221-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  31. Increased osteopontin expression in dendritic cells amplifies IL-17 production by CD4+ T cells in experimental autoimmune encephalomyelitis and in multiple sclerosis.
    Authors: Murugaiyan G, Mittal A, Weiner HL
    J. Immunol., 2008-12-01;181(11):7480-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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