Mouse Integrin alpha 2/CD49b Antibody

Catalog #: MAB1740 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1740
MAB1740-SP

Detection of Integrin alpha 2/CD49b in Mouse Splenocytes by Flow Cytometry.

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Mouse Integrin alpha 2/CD49b Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin alpha 2/CD49b in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Integrin alpha 3, alpha 4, alpha 5, alpha E, or alpha V is observed.

Source

Monoclonal Rat IgG_2B Clone # 235033

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 2/CD49b
Tyr27-Thr1129
Accession # Q62469

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin alpha 2/CD49b in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with (A) Rat Anti-Mouse Integrin a2/CD49b Monoclonal Antibody (Catalog # MAB1740) or (B) Rat IgG2B isotype control antibody (MAB006), followed by APC-conjugated Anti-Rat IgG Secondary Antibody (F0113) and Rat anti-Mouse NKp46 PE-conjugated Monoclonal Antibody (FAB22252P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 2/CD49b

The Integrin alpha 2 subunit, also known as CD49b and VLA-2 alpha subunit, forms a heterodimer with the Integrin alpha 1 (CD29) or alpha 4 (CD104) subunits and binds Laminin.

Entrez Gene IDs

3673 (Human); 16398 (Mouse)

Alternate Names

alpha-2 subunit; CD49b antigen; CD49b; Integrin alpha 2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); ITGA2; VLA 2; VLA-2 alpha