Mouse IL-22 PE-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑22 in Mouse Th17 Splenocytes by Flow Cytometry. Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3e monoclonal antibody (MAB484, 5 µg/mL) plus Goat anti-Mouse CD28 (AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (240-B, 10 ng/mL) Recombinant Mouse IL-23 (1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (MAB485, 10 µg/mL) for 5 days were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (Catalog # FAB554A) and (A) Rat Anti-Mouse IL-22 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC582F) and (A) Rat Anti-Mouse IL-22 PE-conjugated Monoclonal Antibody (IC582P) or (B) isotype control antibody (IC006P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (FC012). View our protocol for Staining Intracellular Molecules.
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Detection of IL‑22 in EL4 cells by Flow Cytometry. EL4 cells were stained with Rat Anti-Mouse IL‑22 PE‑conjugated Monoclonal Antibody (Catalog # IC582P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
Background: IL-22
Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 146 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. The mouse IL-22 gene is localized to chromosome 10. Although it exists as a single copy gene in many mouse strains, the IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.
- Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
- Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
- Dumoutier, L. et al. (2000) PNAS 97:10144.
- Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
Product Datasheets
Citations for Mouse IL-22 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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During acute experimental infection with the reticulotropic Trypanosoma cruzi strain Tulahuen IL-22 is induced IL-23-dependently but is dispensable for protection
Sci Rep, 2016-09-21;6(0):32927.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Intestinal intraepithelial lymphocyte-enterocyte crosstalk regulates production of bactericidal angiogenin 4 by Paneth cells upon microbial challenge.
Authors: Walker C, Hautefort I, Dalton J, Overweg K, Egan C, Bongaerts R, Newton D, Cruickshank S, Andrew E, Carding S
PLoS ONE, 2013-12-17;8(12):e84553.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The Role of IL-15 in Activating STAT5 and Fine-Tuning IL-17A Production in CD4 T Lymphocytes.
Authors: Pandiyan P, Yang X, Saravanamuthu S, Zheng L, Ishihara S
J. Immunol., 2012-09-19;189(9):4237-46.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-22 from conventional NK cells is epithelial regenerative and inflammation protective during influenza infection.
Authors: Kumar P, Thakar M, Ouyang W, Malarkannan S
Mucosal Immunol, 2012-06-27;6(1):69-82.
Species: Mouse
Sample Types: Whole Cells
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Distinct Roles of IL-23 and IL-17 in the Development of Psoriasis-Like Lesions in a Mouse Model.
Authors: Nakajima K, Kanda T, Takaishi M, Shiga T, Miyoshi K, Nakajima H, Kamijima R, Tarutani M, Benson JM, Elloso MM, Gutshall LL, Naso MF, Iwakura Y, DiGiovanni J, Sano S
J. Immunol., 2011-02-23;186(7):4481-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-22-dependent attenuation of T cell-dependent (ConA) hepatitis in herpes virus entry mediator deficiency.
Authors: Wahl C, Wegenka UM, Leithauser F, Schirmbeck R, Reimann J
J. Immunol., 2009-04-15;182(8):4521-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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