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Mouse IL-1 beta /IL-1F2 Antibody

Catalog #: AB-401-NA
25 Citations Datasheet / COA / SDS
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AB-401-NA
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Mouse IL‑1 beta /IL‑1F2 Antibody.
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Mouse IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-1 beta /IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant cotton rat IL-1 beta and recombinant rhesus monkey IL-1 beta is observed, approximately 15% cross-reactivity with recombinant rat IL-1 beta and recombinant rabbit IL-1 beta is observed, and less than 10% cross-reactivity with recombinant canine IL-1 beta, recombinant equine IL-1 beta, recombinant guinea pig IL-1 beta, recombinant porcine IL-1 beta, recombinant feline IL-1 beta, and recombinant human IL-1 beta is observed.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant mouse IL-1 beta /IL-1F2
Val118-Ser269
Accession # P10749
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line [Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272]. The Neutralization Dose (ND50) is typically 2-12 µg/mL in the presence of 10 pg/mL Recombinant Mouse IL‑1 beta /IL‑1F2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Mouse IL‑1 beta /IL‑1F2 Antibody. View Larger

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Mouse IL‑1 beta /IL‑1F2 Antibody. Recombinant Mouse IL-1 beta /IL-1F2 (401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta /IL-1F2 (10 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 beta /IL-1F2 Polyclonal Antibody (Catalog # AB-401-NA). The ND50 is typically 2-12 µg/mL.

Western Blot Detection of Human and Mouse IL-1 beta /IL-1F2 antibody by Western Blot. View Larger

Detection of Human and Mouse IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS for 4 hours and RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IL-1 beta /IL-1F2 Polyclonal Antibody (Catalog # AB-401-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IL-1 beta /IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Mouse IL-1 beta /IL-1F2 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Mouse IL‑1 beta /IL‑1F2 by Simple WesternTM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours, loaded at 0.5 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 40 kDa (as indicated) using 50 µg/mL of Goat Anti-Mouse IL‑1 beta /IL‑1F2 Polyclonal Antibody (Catalog # AB-401-NA). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

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Citations for Mouse IL-1 beta /IL-1F2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Repositioning of the B-Blocker Carvedilol as a Novel Autophagy Inducer That Inhibits the NLRP3 Inflammasome.
    Authors: Wong WT, Li LH, Rao YK et al.
    Front Immunol
  2. Chemo-Protective Potential of Cerium Oxide Nanoparticles against Fipronil-Induced Oxidative Stress, Apoptosis, Inflammation and Reproductive Dysfunction in Male White Albino Rats
    Authors: Hamida Saleh, Atef M. K. Nassar, Ahmed E. Noreldin, Dalia Samak, Norhan Elshony, Lamiaa Wasef et al.
    Molecules
  3. Critical Role for the NLRP3 Inflammasome in Mediating IL-1 beta Production in Shigella sonnei-Infected Macrophages
    Authors: Lan-Hui Li, Tzu-Ling Chen, Hsiao-Wen Chiu, Chung-Hua Hsu, Chien-Chun Wang, Tzu-Ting Tai et al.
    Frontiers in Immunology
  4. Repositioning of the Angiotensin II Receptor Antagonist Candesartan as an Anti-Inflammatory Agent With NLRP3 Inflammasome Inhibitory Activity
    Authors: Lin WY, Li LH, Hsiao YY et al.
    Frontiers in Immunology
  5. Glucosamine inhibits IL-1 beta expression by preserving mitochondrial integrity and disrupting assembly of the NLRP3 inflammasome
    Authors: Hsiao-Wen Chiu, Lan-Hui Li, Chih-Yu Hsieh, Yerra Koteswara Rao, Fang-Hsin Chen, Ann Chen et al.
    Scientific Reports
  6. Downregulation of the Na/K-ATPase Pump by Leptospiral Glycolipoprotein Activates the NLRP3 Inflammasome
    Authors: Sonia Lacroix-Lamandé, Martine Fanton Fanton d’Andon, Eric Michel, Gwenn Ratet, Dana J. Philpott, Stephen E. Girardin et al.
    The Journal of Immunology
  7. Activation of autophagy by inflammatory signals limits IL-1 beta production by targeting ubiquitinated inflammasomes for destruction
    Authors: Chong-Shan Shi, Kevin Shenderov, Ning-Na Huang, Juraj Kabat, Mones Abu-Asab, Katherine A. Fitzgerald et al.
    Nature Immunology
  8. N6-Methyladenosine and Reader Protein YTHDF2 Enhance the Innate Immune Response by Mediating DUSP1 mRNA Degradation and Activating Mitogen-Activated Protein Kinases during Bacterial and Viral Infections
    Authors: J Feng, W Meng, L Chen, X Zhang, A Markazi, W Yuan, Y Huang, SJ Gao
    MBio, 2023-01-10;0(0):e0334922.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. GSK3beta mediates the spatiotemporal dynamics of NLRP3 inflammasome activation
    Authors: S Arumugam, Y Qin, Z Liang, SN Han, SLT Boodapati, J Li, Q Lu, RA Flavell, WZ Mehal, X Ouyang
    Cell Death and Differentiation, 2022-04-27;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  10. Assessing the Association of Mitochondrial Function and Inflammasome Activation in Murine Macrophages Exposed to Select Mitotoxic Tri-Organotin Compounds
    Authors: GM Childers, CA Perry, B Blachut, N Martin, CD Bortner, S Sieber, JL Li, MB Fessler, GJ Harry
    Environmental health perspectives, 2021-04-30;129(4):47015.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. The protective role of proton-sensing TDAG8 in the brain injury in a mouse ischemia reperfusion model
    Authors: K Sato, A Tobo, C Mogi, M Tobo, N Yamane, M Tosaka, H Tomura, DS Im, F Okajima
    Sci Rep, 2020-10-14;10(1):17193.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Mice Lacking the Purinergic Receptor P2X5 Exhibit Defective Inflammasome Activation and Early Susceptibility to Listeria monocytogenes
    Authors: YH Jeong, MC Walsh, J Yu, H Shen, EJ Wherry, Y Choi
    J. Immunol., 2020-06-15;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Coenzyme Q10 protects against burn-induced mitochondrial dysfunction and impaired insulin signaling in mouse skeletal muscle
    Authors: H Nakazawa, K Ikeda, S Shinozaki, S Yasuhara, YM Yu, JAJ Martyn, RG Tompkins, T Yorozu, S Inoue, M Kaneki
    FEBS Open Bio, 2019-01-19;9(2):348-363.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. PtdIns4P on dispersed trans-Golgi network mediates NLRP3 inflammasome activation
    Authors: J Chen, ZJ Chen
    Nature, 2018-11-28;564(7734):71-76.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy.
    Authors: Wang H, Hong L, Huang J, Jiang Q, Tao R, Tan C, Lu N, Wang C, Ahmed M, Lu Y, Liu Z, Shi W, Lai E, Wilcox C, Han F
    Cell Res, 2015-05-22;25(6):674-90.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  16. A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases.
    Authors: Coll R, Robertson A, Chae J, Higgins S, Munoz-Planillo R, Inserra M, Vetter I, Dungan L, Monks B, Stutz A, Croker D, Butler M, Haneklaus M, Sutton C, Nunez G, Latz E, Kastner D, Mills K, Masters S, Schroder K, Cooper M, O'Neill L
    Nat Med, 2015-02-16;21(3):248-55.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Distinct mechanisms of induction of hepatic growth hormone resistance by endogenous IL-6, TNF-alpha, and IL-1beta.
    Authors: Zhao Y, Xiao X, Frank S, Lin H, Xia Y
    Am J Physiol Endocrinol Metab, 2014-06-03;307(2):E186-98.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  18. Interleukin-1beta and fibroblast growth factor receptor 1 cooperate to induce cyclooxygenase-2 during early mammary tumourigenesis.
    Authors: Reed JR, Leon RP, Hall MK, Schwertfeger KL
    Breast Cancer Res., 2009-04-24;11(2):R21.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  19. Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening.
    Authors: Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM
    Bone, 2006-12-21;40(5):1276-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  20. The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1beta.
    Authors: Shchors K, Shchors E, Rostker F, Lawlor ER, Brown-Swigart L, Evan GI
    Genes Dev., 2006-09-15;20(18):2527-38.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  21. A noncanonical function of cGAMP in inflammasome priming and activation
    Authors: Karen V. Swanson, Robert D. Junkins, Cathryn J. Kurkjian, Elizabeth Holley-Guthrie, Avani A. Pendse, Rachid El Morabiti et al.
    Journal of Experimental Medicine
  22. A Synthetic Small Molecule F240B Decreases NLRP3 Inflammasome Activation by Autophagy Induction
    Authors: Wu CH, Gan CH, Li LH et al.
    Frontiers in Immunology
  23. Inflammasome-mediated GSDMD activation facilitates escape of Candida albicans from macrophages
    Authors: X Ding, H Kambara, R Guo, A Kanneganti, M Acosta-Zal, J Li, F Liu, T Bei, W Qi, X Xie, W Han, N Liu, C Zhang, X Zhang, H Yu, L Zhao, F Ma, JR Köhler, HR Luo
    Nature Communications, 2021-11-18;12(1):6699.
  24. NLRP3 Inflammasome Mediates Dormant Neutrophil Recruitment following Sterile Lung Injury and Protects against Subsequent Bacterial Pneumonia in Mice
    Authors: Xiaoli Tian, He Sun, Amy-Jo Casbon, Edward Lim, Kevin P. Francis, Judith Hellman et al.
    Frontiers in Immunology

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