Mouse IL-1 beta /IL-1F2 Antibody

Name: Mouse IL-1 beta /IL-1F2 Antibody
Brand: R&D Systems
SKU: MAB4012
Rating: 5 (1 reviews)

Catalog #: MAB4012
12 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4012
MAB4012-SP

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse IL‑1 beta /IL‑1F2 Antibody.

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Citations (12)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-1 beta /IL-1 F2 in direct ELISAs and Western blots. In Western blots, shows 100% cross-reactivity with recombinant rat (rr) IL‑1 beta and rcrIL‑1 beta, 15%‑35 % with rhIL‑1 beta and rpIL‑1 beta, and no cross-reactivity with rmIL-1 alpha, rmIL-18, or rhIL-1 beta rp.

Source

Monoclonal Hamster IgG Clone # B122

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse IL-1 beta
Accession # P10749

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse IL‑1 beta /IL‑1F2 (Catalog # 401-ML)

Immunoprecipitation

Hogquist, K.A. et al. (1991) J. Immunol. 146:1534.

Neutralization

Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 0.3-1.5 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 beta /IL‑1F2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse IL‑1 beta /IL‑1F2 Antibody. Recombinant Mouse IL-1 beta /IL-1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB4012). The ND₅₀ is typically 0.3-1.5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name

Interleukin 1 beta

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Alternate Names

catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IL-1 beta /IL-1F2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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PINK1 and PARK2 Suppress Pancreatic Tumorigenesis through Control of Mitochondrial Iron-Mediated Immunometabolism
Authors: Li C, Zhang Y, Cheng X et al.
Dev. Cell
Caspase-1 induces smooth muscle cell growth in hypoxia-induced pulmonary hypertension
Authors: Camilla Udjus, Fadila T. Cero, Bente Halvorsen, Dina Behmen, Cathrine R. Carlson, Bård A. Bendiksen et al.
American Journal of Physiology-Lung Cellular and Molecular Physiology
IL-1 beta drives production of FGF-23 at the onset of chronic kidney disease in mice
Authors: Mcknight Q, Jenkins S, Li X et Al.
J. Bone Miner. Res.
Minocycline reverses IL-17A/TRAF3IP2-mediated p38 MAPK/NF-kappaB/iNOS/NO-dependent cardiomyocyte contractile depression and death
Authors: T Yoshida, NA Das, AJ Carpenter, R Izadpanah, SA Kumar, S Gautam, SB Bender, U Siebenlist, B Chandrasek
Cell. Signal., 2020-06-15;73(0):109690.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Cells
Applications: Neutralization, Western Blot
Mycobacterium tuberculosis Limits Host Glycolysis and IL-1&beta by Restriction of PFK-M via MicroRNA-21
Authors: EE Hackett, H Charles-Me, SM O'Leary, LE Gleeson, N Muñoz-Wolf, S Case, A Wedderburn, DGW Johnston, MA Williams, A Smyth, M Ouimet, KJ Moore, EC Lavelle, SC Corr, SV Gordon, J Keane, FJ Sheedy
Cell Rep, 2020-01-07;30(1):124-136.e4.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Nicotinamide phosphoribosyltransferase aggravates inflammation and promotes atherosclerosis in ApoE knockout mice
Authors: YY Kong, GQ Li, WJ Zhang, X Hua, CC Zhou, TY Xu, ZY Li, P Wang, CY Miao
Acta Pharmacol. Sin., 2019-03-04;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Cholinergic anti-inflammatory pathway inhibits neointimal hyperplasia by suppressing inflammation and oxidative stress
Authors: DJ Li, H Fu, J Tong, YH Li, LF Qu, P Wang, FM Shen
Redox Biol, 2017-11-16;15(0):22-33.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Key Role of the Scavenger Receptor MARCO in Mediating Adenovirus Infection and Subsequent Innate Responses of Macrophages
Authors: MD Maler, PJ Nielsen, N Stichling, I Cohen, Z Ruzsics, C Wood, P Engelhard, M Suomalaine, I Gyory, M Huber, J Müller-Que, WWA Schamel, S Gordon, T Jakob, SF Martin, W Jahnen-Dec, UF Greber, MA Freudenber, G Fejer
MBio, 2017-08-01;8(4):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Cigarette smoke-induced accumulation of lung dendritic cells is interleukin-1alpha-dependent in mice.
Authors: Botelho F, Nikota J, Bauer C, Morissette M, Iwakura Y, Kolbeck R, Finch D, Humbles A, Stampfli M
Respir Res, 2012-09-19;13(0):81.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
IL-1alpha/IL-1R1 expression in chronic obstructive pulmonary disease and mechanistic relevance to smoke-induced neutrophilia in mice.
Authors: Botelho F, Bauer C, Finch D, Nikota J, Zavitz C, Kelly A, Lambert K, Piper S, Foster M, Goldring J, Wedzicha J, Bassett J, Bramson J, Iwakura Y, Sleeman M, Kolbeck R, Coyle A, Humbles A, Stampfli M
PLoS ONE, 2011-12-06;6(12):e28457.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation.
Authors: Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN
J. Immunol., 2011-09-19;187(9):4835-43.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Ex vivo culture of mouse skin activates an interleukin 1 alpha‐dependent inflammatory response
Authors: Hong‐Ming Zhou, Radomir M. Slominski, Leroy J. Seymour, Maria C. Bell, Priya Dave, Joseph Atumonye et al.
Experimental Dermatology