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Mouse IL-1 alpha /IL-1F1 Antibody

Catalog #: AF-400-NA
30 Citations 3 Reviews Datasheet / COA / SDS
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AF-400-NA
AF-400-SP
Detection of Recombinant Mouse and Rat IL‑1 alpha /IL‑1F1 by Western Blot.
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Product Details
Citations (30)
FAQs
Supplemental Products
Reviews (3)
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Mouse IL-1 alpha /IL-1F1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑1 alpha /IL‑1F1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant rat IL‑1 alpha  and recombinant cotton rat IL‑1 alpha  is observed and less than 1% cross-reactivity with recombinant human IL‑1 alpha is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL‑1 alpha /IL‑1F1
Ser6-Ser161
Accession # Q62161
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
3-15 µg/mL
Perfusion fixed frozen sections of mouse liver and immersion fixed paraffin-embedded sections of mouse liver
Neutralization
Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.002-0.004 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 alpha /IL‑1F1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Mouse and Rat IL-1a/IL-1F1 antibody by Western Blot. View Larger

Detection of Recombinant Mouse and Rat IL‑1 alpha /IL‑1F1 by Western Blot. Western blot shows 50 ng of Recombinant Mouse IL-1a/IL-1F1 (400-ML), Recombinant Human IL-1a/IL-1F1 (200-LA) and Recombinant Rat IL-1a/IL-1F1 (500-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for IL-1a/IL-1F1 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry IL-1a/IL-1F1 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr). View Larger

IL‑1 alpha /IL‑1F1 in Mouse Liver. IL-1a/IL-1F1 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Neutralization Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutral-ization by Mouse IL‑1 alpha /IL‑1F1 Antibody. View Larger

Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutral-ization by Mouse IL‑1 alpha /IL‑1F1 Antibody. Recombinant Mouse IL-1a/IL-1F1 (400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA). The ND50 is typically 0.002‑0.004 µg/mL.

Immunohistochemistry View Larger

Detection of IL‑1 alpha /IL‑1F1 in Mouse Liver. IL‑1 alpha /IL‑1F1 was detected in immersion fixed paraffin-embedded sections of Mouse Liver using Goat Anti-Mouse IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry/ Immunofluorescence Detection of Mouse IL-1 alpha/IL-1F1 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Mouse IL-1 alpha/IL-1F1 by Immunocytochemistry/ Immunofluorescence Experimental stroke-induced IL-1 expression is increased in the brain after LPS administration and anaphylaxis. Quantification of IL-1 alpha -positive cells (A) and granulocytes (B) in the ipsilateral hemisphere. C. Immunofluorescence shows IL-1 alpha -positive cells (green), granulocytes (red) and tomato lectin-positive blood vessels (blue) and microglia/macrophage cells in the ipsilateral cerebral cortex and thalamus after 24 h reperfusion. Perivascular cells in the cerebral cortex (i) and parenchymal cells in the thalamus (ii) expressing IL-1 alpha after MCAo are abundant in mice with anaphylaxis and LPS treatment, respectively. D. In situ hybridization reveals increased number of cells expressing IL-1 beta mRNA in the ischaemic cerebral cortex of mice 24 h after MCAo with anaphylaxis (Ova+A) compared to MCAo and Ova sensitization (Ova). E. Silver grains indicate the presence of IL-1 beta mRNA in Ova and Ova+A mice after MCAo (counterstained with cresyl violet). Inserts show higher magnification of IL-1 beta mRNA containing cells indicated by arrowheads. Data are representative of 4-5 mice in each group. One-way ANOVA followed by Bonferroni's comparison (P < 0.05 vs * Control, # Ova, $ Ova+A). Scale bars: Cii - 20 μm, C - 50 μm, E - 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22114895), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse IL-1 alpha/IL-1F1 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Mouse IL-1 alpha/IL-1F1 by Immunocytochemistry/ Immunofluorescence Experimental stroke-induced IL-1 expression is increased in the brain after LPS administration and anaphylaxis. Quantification of IL-1 alpha -positive cells (A) and granulocytes (B) in the ipsilateral hemisphere. C. Immunofluorescence shows IL-1 alpha -positive cells (green), granulocytes (red) and tomato lectin-positive blood vessels (blue) and microglia/macrophage cells in the ipsilateral cerebral cortex and thalamus after 24 h reperfusion. Perivascular cells in the cerebral cortex (i) and parenchymal cells in the thalamus (ii) expressing IL-1 alpha after MCAo are abundant in mice with anaphylaxis and LPS treatment, respectively. D. In situ hybridization reveals increased number of cells expressing IL-1 beta mRNA in the ischaemic cerebral cortex of mice 24 h after MCAo with anaphylaxis (Ova+A) compared to MCAo and Ova sensitization (Ova). E. Silver grains indicate the presence of IL-1 beta mRNA in Ova and Ova+A mice after MCAo (counterstained with cresyl violet). Inserts show higher magnification of IL-1 beta mRNA containing cells indicated by arrowheads. Data are representative of 4-5 mice in each group. One-way ANOVA followed by Bonferroni's comparison (P < 0.05 vs * Control, # Ova, $ Ova+A). Scale bars: Cii - 20 μm, C - 50 μm, E - 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22114895), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 alpha/IL-1F1

IL-1 is a name that designates two proteins, IL-1 alpha  and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta, designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.

IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti‑inflammatory agents such as glucocorticoids, IL-4 and IL-13.

Long Name
Interleukin 1 alpha
Entrez Gene IDs
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)
Alternate Names
BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha

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Citations for Mouse IL-1 alpha /IL-1F1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. T. gondii infection induces IL-1R dependent chronic cachexia and perivascular fibrosis in the liver and skeletal muscle.
    Authors: Melchor, S J, Hatter, J A Et al.
    Sci Rep
  2. Toll-like receptor 2 orchestrates a tumor suppressor response in non-small cell lung cancer
    Authors: FR Millar, A Pennycuick, M Muir, A Quintanill, P Hari, E Freyer, P Gautier, A Meynert, G Grimes, CS Coll, S Zdral, S Victorelli, VH Teixeira, J Connelly, JF Passos, MA Ros, WAH Wallace, MC Frame, AH Sims, L Boulter, SM Janes, S Wilkinson, JC Acosta
    Cell Reports, 2022-11-08;41(6):111596.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. The alarmin interleukin-1? triggers secondary degeneration through reactive astrocytes and endothelium after spinal cord injury
    Authors: Bretheau F, Castellanos-Molina A, B�langer D et al.
    Nature Communications
  4. Itaconate and fumarate derivatives inhibit priming and activation of the canonical NLRP3 inflammasome in macrophages
    Authors: Christopher Hoyle, Jack P. Green, Stuart M. Allan, David Brough, Eloise Lemarchand
    Immunology
  5. Augmented secretion of IL-1&alpha from mouse oral squamous cell carcinoma (OSCC) cells caused by serum deprivation and hypoxia promotes immune suppressive activity of mesenchymal stromal cells
    Authors: A Matsunami, M Mizuno-Kam, H Kawaki, E Takayama, K Ueno, M Ando, H Morimoto-I, Y Muramatsu, S Sumitomo, N Kondoh
    Journal of oral biosciences, 2021-06-18;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Functional Assay
  6. Gasdermin D mediates the maturation and release of IL-1&alpha downstream of inflammasomes
    Authors: K Tsuchiya, S Hosojima, H Hara, H Kushiyama, MR Mahib, T Kinoshita, T Suda
    Cell Reports, 2021-03-23;34(12):108887.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Macrophages potentiate STAT3 signaling in skeletal muscles and regulate pancreatic cancer cachexia
    Authors: Surendra K. Shukla, Spas D. Markov, Kuldeep S. Attri, Enza Vernucci, Ryan J. King, Aneesha Dasgupta et al.
    Cancer Letters
  8. Ptpn6 inhibits caspase-8- and Ripk3/Mlkl-dependent inflammation
    Authors: M Speir, CJ Nowell, AA Chen, JA O'Donnell, IS Shamie, PR Lakin, AA D'Cruz, RO Braun, JJ Babon, RS Lewis, M Bliss-More, I Shlomovitz, S Wang, LH Cengia, AI Stoica, R Hakem, MA Kelliher, LA O'Reilly, H Patsiouras, KE Lawlor, E Weller, NE Lewis, AW Roberts, M Gerlic, BA Croker
    Nat. Immunol., 2019-12-09;21(1):54-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  9. Developmentally Regulated Innate Immune NF kappa B Signaling Mediates IL-1 alpha Expression in the Perinatal Murine Lung
    Authors: Brittany Butler, Robyn De Dios, Leanna Nguyen, Sarah McKenna, Sankar Ghosh, Clyde J. Wright
    Frontiers in Immunology
  10. The three cytokines IL-1?, IL-18, and IL-1? share related but distinct secretory routes
    Authors: VS Tapia, MJD Daniels, P Palazón-Ri, M Dewhurst, NM Luheshi, J Rivers-Aut, J Green, E Redondo-Ca, P Kaldis, G Lopez-Cast, D Brough
    J. Biol. Chem., 2019-04-02;294(21):8325-8335.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  11. Tacrolimus interacts with voriconazole to reduce the severity of fungal keratitis by suppressing IFN-related inflammatory responses and concomitant FK506 and voriconazole treatment suppresses fungal keratitis
    Authors: J Zhong, L Peng, B Wang, H Zhang, S Li, R Yang, Y Deng, H Huang, J Yuan
    Mol. Vis., 2018-03-04;24(0):187-200.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. HpARI Protein Secreted by a Helminth Parasite Suppresses Interleukin-33
    Authors: Megan Osbourn, Dinesh C. Soares, Francesco Vacca, E. Suzanne Cohen, Ian C. Scott, William F. Gregory et al.
    Immunity
  13. TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects
    Authors: MK Choo, Y Sano, C Kim, K Yasuda, XD Li, X Lin, M Stenzel-Po, L Alexopoulo, S Ghosh, E Latz, IR Rifkin, ZJ Chen, GC Stewart, H Chong, JM Park
    J. Exp. Med., 2017-04-11;214(5):1297-1311.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Interleukin-1 and estrogen protect against disseminating dentoalveolar infections
    Authors: H Youssef, P Stashenko
    Int J Oral Sci, 2017-03-01;9(1):16-23.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  15. Sustained, localized salicylic acid delivery enhances diabetic bone regeneration via prolonged mitigation of inflammation
    Authors: Weiling Yu, Stephan Bien-Aime, Marcelo Mattos, Sarah Alsadun, Keisuke Wada, Sarah Rogado et al.
    Journal of Biomedical Materials Research Part A
  16. Caspase-1-independent IL-1 release mediates blister formation in autoantibody-induced tissue injury through modulation of endothelial adhesion molecules.
    Authors: Sadeghi H, Lockmann A, Hund A, Samavedam U, Pipi E, Vafia K, Hauenschild E, Kalies K, Pas H, Jonkman M, Iwata H, Recke A, Schon M, Zillikens D, Schmidt E, Ludwig R
    J Immunol, 2015-03-20;194(8):3656-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  17. AIM2 and NLRC4 inflammasomes contribute with ASC to acute brain injury independently of NLRP3.
    Authors: Denes A, Coutts G, Lenart N, Cruickshank S, Pelegrin P, Skinner J, Rothwell N, Allan S, Brough D
    Proc Natl Acad Sci U S A, 2015-03-16;112(13):4050-5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  18. IL-1alpha signaling is critical for leukocyte recruitment after pulmonary Aspergillus fumigatus challenge.
    Authors: Caffrey A, Lehmann M, Zickovich J, Espinosa V, Shepardson K, Watschke C, Hilmer K, Thammahong A, Barker B, Rivera A, Cramer R, Obar J
    PLoS Pathog, 2015-01-28;11(1):e1004625.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  19. Dendritic cell IL-1alpha and IL-1beta are polyubiquitinated and degraded by the proteasome.
    Authors: Ainscough J, Frank Gerberick G, Zahedi-Nejad M, Lopez-Castejon G, Brough D, Kimber I, Dearman R
    J Biol Chem, 2014-11-04;289(51):35582-92.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Cytokine signaling by grafted neuroectodermal stem cells rescues motoneurons destined to die
    Authors: Krisztián Pajer, Georg A. Feichtinger, Gábor Márton, Sonja Sabitzer, Dieter Klein, Heinz Redl et al.
    Experimental Neurology
  21. Interleukin-1 deficiency prolongs ovarian lifespan in mice.
    Authors: Uri-Belapolsky S, Shaish A, Eliyahu E, Grossman H, Levi M, Chuderland D, Ninio-Many L, Hasky N, Shashar D, Almog T, Kandel-Kfir M, Harats D, Shalgi R, Kamari Y
    Proc Natl Acad Sci U S A, 2014-08-11;111(34):12492-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  22. The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines.
    Authors: Asrat S, Dugan A, Isberg R
    PLoS Pathog, 2014-07-24;10(7):e1004229.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. I kappa B epsilon Is a Key Regulator of B Cell Expansion by Providing Negative Feedback on cRel and RelA in a Stimulus-Specific Manner
    Authors: Bryce N. Alves, Rachel Tsui, Jonathan Almaden, Maxim N. Shokhirev, Jeremy Davis-Turak, Jessica Fujimoto et al.
    The Journal of Immunology
  24. Genetic and cellular evidence of decreased inflammation associated with reduced incidence of posttraumatic arthritis in MRL/MpJ mice
    Authors: John S. Lewis, Bridgette D. Furman, Evan Zeitler, Janet L. Huebner, Virginia B. Kraus, Farshid Guilak et al.
    Arthritis & Rheumatism
  25. Interleukin-1 alpha expression precedes IL-1 beta after ischemic brain injury and is localised to areas of focal neuronal loss and penumbral tissues
    Authors: Nadia M Luheshi, Krisztina J Kovács, Gloria Lopez-Castejon, David Brough, Adam Denes
    Journal of Neuroinflammation
  26. Systemic inflammatory challenges compromise survival after experimental stroke via augmenting brain inflammation, blood- brain barrier damage and brain oedema independently of infarct size
    Authors: Ádám Dénes, Szilamér Ferenczi, Krisztina J Kovács
    Journal of Neuroinflammation
  27. IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation.
    Authors: Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN
    J. Immunol., 2011-09-19;187(9):4835-43.
    Species: Mouse
    Sample Types: Cell Lysates, In Vivo, Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr, Neutralization, Western Blot
  28. Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a mechanism for enhanced IL-1beta in type 2 diabetes.
    Authors: Masters SL, Dunne A, Subramanian SL, Hull RL, Tannahill GM, Sharp FA, Becker C, Franchi L, Yoshihara E, Chen Z, Mullooly N, Mielke LA, Harris J, Coll RC, Mills KH, Mok KH, Newsholme P, Nunez G, Yodoi J, Kahn SE, Lavelle EC, O'Neill LA
    Nat. Immunol., 2010-09-12;11(10):897-904.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening.
    Authors: Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM
    Bone, 2006-12-21;40(5):1276-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  30. Membrane-bound Interleukin-1 alpha mediates leukocyte adhesion during atherogenesis
    Species: Mouse
    Sample Types: Cell Fractions
    Applications: Western Blot

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Reviews for Mouse IL-1 alpha /IL-1F1 Antibody

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Mouse IL-1 alpha /IL-1F1 Antibody
By Anonymous on 05/04/2018
Application: IHC Sample Tested: Breast cancer cells Species: Mouse
Mouse IL-1 alpha /IL-1F1 Antibody
By Anonymous on 04/26/2018
Application: IHC Sample Tested: Pancreatic cancer cells Species: Mouse
Mouse IL-1 alpha /IL-1F1 Antibody
By Anonymous on 12/01/2017
Application: WB Sample Tested: Liver tissue,Spleen tissue Species: Mouse