Mouse IL-18 R beta /IL-1 R7 Biotinylated Antibody

Catalog #: BAF1520
3 Citations Datasheet / COA / SDS

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BAF1520

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Citations (3)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-18 R beta /IL-1 R7 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-18 R beta in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human (rh) IL-18 R beta is observed, 10% cross-reactivity with rhIL-1 R9 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) IL-1 R8, recombinant rat IL-1 R6, rhIL-1 R3, rmIL-1 R1, rmIL-1 RII, and rmIL-18 R is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R beta /IL‑1 R7
Phe20-Glu356
Accession # Q9Z2B1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑18 R beta /IL‑1 R7

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18 R beta/IL-1 R7

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL-18R alpha (IL-1 R5) and IL-18R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL-18R alpha by itself binds IL-18 with low-affinity and IL-18R beta does not bind IL-18 in vitro, co-expression of IL-18R alpha and IL-18R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a aa signal peptide, a aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18R beta parallels that of IL-18R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.

References

Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.

Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.

Long Name

Interleukin 18 Receptor beta

Entrez Gene IDs

8807 (Human); 16174 (Mouse)

Alternate Names

Accessory protein-like; AcPL; CD218 antigen-like family member B; CD218b antigen; CD218b; CDw218b; IL-1 R7; IL-18 R beta; IL-18 receptor accessory protein; IL-18 receptor beta; IL18R beta; IL-18RAcP; IL18RAP; IL18RB; IL-18Rb; IL-18Rbeta; IL-18R-beta; IL-1R accessory protein-like; IL1R7; IL-1R7; IL-1R-7; IL-1RAcPL; interleukin 18 receptor accessory protein; Interleukin-1 receptor 7; interleukin-18 receptor accessory protein; Interleukin-18 receptor accessory protein-like; Interleukin-18 receptor beta; MGC120589; MGC120590

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse IL-18 R beta /IL-1 R7 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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MCMV triggers ROS/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing loss
Authors: W Zhuang, C Wang, X Shi, S Qiu, S Zhang, B Xu, M Chen, W Jiang, H Dong, Y Qiao
Int. J. Mol. Med., 2018-03-06;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Constitutive and LPS-regulated expression of interleukin-18 receptor beta variants in the mouse brain
Authors: Silvia Alboni, Claudia Montanari, Cristina Benatti, Johanna M.C. Blom, Maria Luisa Simone, Nicoletta Brunello et al.
Brain, Behavior, and Immunity
Protection of CD8+ T cells from activation-induced cell death by IL-18.
Authors: Li W, Kashiwamura S, Ueda H, Sekiyama A, Okamura H
J. Leukoc. Biol., 2007-03-30;82(1):142-51.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry