Mouse IL-17RD/SEF Antibody Summary
Gly28-Arg299
Accession # Q8JZL1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑17 RD/SEF in HEK293 Human Cell Line transfected with Mouse IL-17 RD/SEF by Flow Cytometry. HEK293 human cell line transfected with mouse IL-17 RD/SEF was stained with Goat Anti-MouseIL-17 RD/SEF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2276, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
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Detection of IL‑17 RD/SEF in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse IL-17 RD/SEF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2276, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17RD/SEF
Interleukin -17 receptor D (IL-17 RD), also known as SEF (similar expression to FGFs), is a type I transmembrane protein that is found in both the cytoplasm and plasma membrane (1‑5). The gene for this protein belongs to a synexpression group originally identified in zebrafish and SEF is expressed along with FGF-3, -8, sprouty-2 (SPRY2) and SPRY4 (6, 7). Due to the presence of an alternate start site, there is one transcript that potentially gives rise to two isoforms. The first is a full-length long form and the second an N-terminally truncated form (2, 5). The significance and expression pattern of the short form are uncertain. The membrane‑bound long form of mouse IL-17 RD is synthesized as a 738 amino acid (aa) precursor protein with a putative 27 aa signal peptide, a 272 aa extracellular domain, a 20 aa transmembrane segment and a 419 aa cytoplastic domain (5). The extracellular domain contains one Ig-like domain and a fibronectin type III motif. The cytoplasmic domain shares homology with the intracellular domains of IL-17 receptor family members and shows one TIR (Toll/IL-1 Receptor) domain and a putative TRAF6-binding motif (2). Natural IL-17 RD has been shown to form homomultimeric complexes (3). The full-length IL-17 RD isoform is expressed in most adult tissues and during embryonic development (3, 5). Functionally, IL-17 RD has been shown to be an inhibitor of FGF signaling. The molecule’s extracellular domain does not seem to be involved. There is an interaction between the intracellular domains of FGFR1/2 and IL-17 RD that blocks ERK dissociation from MEK, thereby interfering with downstream ERK activation of nuclear Elk-1 (8). IL-17 RD has also been reported to interact with TAK1 and induce JNK activation and apoptosis (9). Ligands that interact with the extracellular domain of IL-17 RD have not been identified.
- Furthauer, M. et al. (2002) Nat. Cell Biol. 4:170.
- Xiong, S. et al. (2003) J. Biol. Chem. 278:50273.
- Yang, R-B. et al. (2003) J. Biol. Chem. 278:33232.
- Preger, E. et al. (2003) Proc. Natl. Acad. Sci. USA 101:1229.
- Lin, W. et al. (2002) Mech. Dev. 113:163.
- Tsang, M. et al. (2002) Nat. Cell Biol. 4:165.
- Kovalenko, D. et al. (2003) J. Biol. Chem. 278:14087.
- Torii, S. et al. (2004) Dev. Cell 7:33.
- Yang, X. et al. (2004) J. Biol. Chem. 279:38099.
Product Datasheets
Citations for Mouse IL-17RD/SEF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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CMTM4 is a subunit of the IL-17 receptor and mediates autoimmune pathology
Authors: D Knizkova, M Pribikova, H Draberova, T Semberova, T Trivic, A Synackova, A Ujevic, J Stefanovic, A Drobek, M Huranova, V Niederlova, O Tsyklauri, A Neuwirth, J Tureckova, O Stepanek, P Draber
Nature Immunology, 2022-10-21;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Soluble Endoglin Stimulates Inflammatory and Angiogenic Responses in Microglia That Are Associated with Endothelial Dysfunction
Authors: ES Park, S Kim, DC Yao, JPJ Savarraj, HA Choi, PR Chen, E Kim
International Journal of Molecular Sciences, 2022-01-22;23(3):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Mutations in FGF17, IL17RD, DUSP6, SPRY4, and FLRT3 are identified in individuals with congenital hypogonadotropic hypogonadism.
Authors: Miraoui H, Dwyer A, Sykiotis G, Plummer L, Chung W, Feng B, Beenken A, Clarke J, Pers T, Dworzynski P, Keefe K, Niedziela M, Raivio T, Crowley W, Seminara S, Quinton R, Hughes V, Kumanov P, Young J, Yialamas M, Hall J, Van Vliet G, Chanoine J, Rubenstein J, Mohammadi M, Tsai P, Sidis Y, Lage K, Pitteloud N
Am J Hum Genet, 2013-05-02;92(5):725-43.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling.
Authors: Rong Z, Wang A, Li Z, Ren Y, Cheng L, Li Y, Wang Y, Ren F, Zhang X, Hu J, Chang Z
Cell Res., 2009-02-01;19(2):208-15.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot
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