Mouse IL-17/IL-17A PerCP-conjugated Antibody

Catalog #: IC421C
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IC421C

Detection of IL‑17/IL‑17A in Mouse splenocytes by Flow Cytometry.

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All IL-17/IL-17A Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-17/IL-17A PerCP-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human IL-17A and recombinant mouse (rm) IL-17F is observed and less than 1% cross-reactivity with rmIL-17B, rmIL-17C, rmIL‑17D, and rmIL-17E is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse IL‑17
Thr22-Ala158
Accession # Q62386

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

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Detection of IL‑17/IL‑17A in Mouse splenocytes by Flow Cytometry. Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-mouse CD3 Monoclonal antibody (10 μg/ml; MAB484), Goat anti-mouse CD28 Polyclonal Antibody (5 μg/mL; AF483), TGF-beta (10 ng/mL; 100-B), Recombinant Mouse IL-23 (20 ng/mL; 1887-ML), Recombinant Mouse IL-6 (40 ng/mL; 406-ML), and Recombinant MouseIL-1 beta (10 ng/mL; 401-ML) for 6 days were stained with (A) Goat Anti-Mouse IL-17/IL-17A PerCP-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC421C) or (B) control antibody (IC108C) and Rat anti-Mouse CD4 PE-conjugated Monoclonal Antibody (FAB554P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.

Intracellular Staining by Flow Cytometry

Detection of IL-17/IL-17A antibody in EL-4 Mouse Cell Line antibody by Flow Cytometry.

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Detection of IL‑17/IL‑17A in EL‑4 Mouse Cell Line by Flow Cytometry. LPS activated EL-4 mouse lymphoblast cell line was stained with Goat Anti-Mouse IL-17/IL-17A PerCP-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC421C, filled histogram) or isotype control antibody (Catalog # IC108C, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-17/IL-17A

Interleukin 17, also known as IL-17A and CTLA-8, was initially identified as a 17 kDa, secreted T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 25 amino acid residue signal peptide that is cleaved to yield the 133 aa residue mature IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 is also known to form a heterodimer with IL-17F. At the amino acid level, mIL-17 shows 62% and 87% aa sequence identity with human and rat IL-17, respectively. The receptor for the IL-17A homodimer and IL-17A:F heterodimer is reported to be a combinationof IL-17 RA and IL-17 RC, with a possible contribution by IL-17 RD. The expression of IL-17 is widespread, and found associated with LTi cells, B cells, gamma δ T cells, CD4⁺ Th17 cells, iNKT cells, neutrophils, intestinal Paneth cells, Type I ILCs and CD8⁺ T_c17 cells. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation, the preservation of intestinal mucosal integrity, and the induction of antimicrobial peptides by epithelium.

Long Name

Interleukin 17

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Alternate Names

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

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Citation for Mouse IL-17/IL-17A PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Maternal high-fat diet results in microbiota-dependent expansion of ILC3s in mice offspring
Authors: ST Babu, X Niu, M Raetz, RC Savani, LV Hooper, J Mirpuri
JCI Insight, 2018-10-04;3(19):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry