Home / IL-12/IL-23 p40 / Mouse IL-12/IL-23 p40 Antibody

Mouse IL-12/IL-23 p40 Antibody

Catalog #: MAB4991
9 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4991-SP
MAB4991-100
MAB4991-500
Cell Proliferation Induced by IL‑12 and Neutralization by Mouse IL‑12/IL‑23 p40 Antibody.
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Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-12/IL-23 p40 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects the mouse IL-12/IL-23 p40 subunit. Polymorphisms exist in the mouse p40 sequences [Ymer, S. et al. (2002) Genes and Immunity 3:151.].
Source
Monoclonal Rat IgG2A Clone # C17.8
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant mouse IL-12 p70 heterodimer
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IL-12/IL-23 p40 Homodimer (Catalog # 499-ML)
under non-reducing conditions only

Mouse IL-12/IL-23 p40 Nonallele-specific Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL‑12/IL‑23 p40 Biotinylated Antibody (Catalog # BAF499)

Standard: Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein (Catalog # 499-ML)

Neutralization
Measured by its ability to neutralize IL‑12-induced proliferation in PHA-activated mouse splenocytes. The Neutralization Dose (ND50) is typically 0.04-0.3 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑12.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑12 and Neutralization by Mouse IL‑12/IL‑23 p40 Antibody. View Larger

Cell Proliferation Induced by IL‑12 and Neutralization by Mouse IL‑12/IL‑23 p40 Antibody. Recombinant Mouse IL-12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-12/IL-23 p40 Monoclonal Antibody (Catalog # MAB4991). The ND50 is typically 0.04-0.3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12/IL-23 p40

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 has multiple effects on T cells and NK cells and is a key mediator in the development of Th1 cells.

IL-12 is a heterodimeric cytokine containing two disulfide-linked subunits, p35 and p40. Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. Although mouse IL-12 is active on human or mouse IL-12 responsive cells, human IL-12 is not active on mouse cells.

The disulfide-linked mouse p40 homodimer can bind to IL-12 receptors and is an antagonist of IL-12 activities in vitro. The mouse p40 monomer is at least ten times less active than the homodimer as an IL-12 antagonist. At the present time, the existence and the physiological role of mouse p40 homodimer in vivo remains to be determined.

Long Name
Interleukin 12/Interleukin 23 p40
Entrez Gene IDs
3593 (Human); 16160 (Mouse); 64546 (Rat); 397076 (Porcine); 403976 (Canine); 102124858 (Cynomolgus Monkey); 493741 (Feline); 694747 (Rhesus Macaque)
Alternate Names
CLMF p40; CLMF; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 p40; IL-12 subunit p40; IL12B; IL-12B; IL-12BNK cell stimulatory factor chain 2; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NKSF; NKSF2; NKSF2IL12, subunit p40

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Citations for Mouse IL-12/IL-23 p40 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Transgenic viral expression of PH-20, IL-12, and sPD1-Fc enhances immune cell infiltration and anti-tumor efficacy of an oncolytic virus
    Authors: Soon-Oh Hong, Joonsung Kim, Sungmin Lee, Jaeil Shin, Hwanjun Choi, Eunjin Lee et al.
    Mol Ther Oncolytics
  2. IFN-?-Expressing Th17 Cells Are Required for Development of Severe Ocular Surface Autoimmunity
    Authors: Y Chen, SK Chauhan, C Shao, M Omoto, T Inomata, R Dana
    J. Immunol., 2017-06-21;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections.
    Authors: Ohama H, Asai A, Ito I, Suzuki S, Kobayashi M, Higuchi K, Suzuki F
    Am J Pathol, 2014-12-06;185(2):420-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  4. Co-adjuvant effects of retinoic acid and IL-15 induce inflammatory immunity to dietary antigens.
    Authors: DePaolo RW, Abadie V, Tang F
    Nature, 2011-02-09;471(7337):220-4.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Neutralization
  5. The pro-Th2 cytokine IL-33 directly interacts with invariant NKT and NK cells to induce IFN-gamma production.
    Authors: Bourgeois E, Van LP, Samson M, Diem S, Barra A, Roga S, Gombert JM, Schneider E, Dy M, Gourdy P, Girard JP, Herbelin A
    Eur. J. Immunol., 2009-04-01;39(4):1046-55.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  6. TSU68 prevents liver metastasis of colon cancer xenografts by modulating the premetastatic niche.
    Authors: Yamamoto M, Kikuchi H, Ohta M, Kawabata T, Hiramatsu Y, Kondo K, Baba M, Kamiya K, Tanaka T, Kitagawa M, Konno H
    Cancer Res., 2008-12-01;68(23):9754-62.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages.
    Authors: An HJ, Jeong HJ, Um JY, Kim HM, Hong SH
    J Ethnopharmacol, 2006-03-10;106(3):418-24.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  8. 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
    Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
    Blood, 2004-05-13;104(5):1404-10.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  9. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12.
    Authors: Oppmann, B, Lesley, R, Blom, B, Timans, J C, Xu, Y, Hunte, B, Vega, F, Yu, N, Wang, J, Singh, K, Zonin, F, Vaisberg, E, Churakova, T, Liu, M, Gorman, D, Wagner, J, Zurawski, S, Liu, Y, Abrams, J S, Moore, K W, Rennick, D, de Waal-Malefyt, R, Hannum, C, Bazan, J F, Kastelein, R A
    Immunity, 2000-11-01;13(5):715-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Mouse IL-12/IL-23 p40 Antibody

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Mouse IL-12/IL-23 p40 Antibody
By Anonymous on 11/09/2021
Application: WB Sample Tested: Dendritic cells Species: Mouse
Western Blot Mouse IL-12/IL-23 p40 Antibody MAB4991
Mouse IL-12/IL-23 p40 Antibody
By Anonymous on 04/09/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Melanoma tissue Species: Human
Immunocytochemistry/Immunofluorescence Mouse IL-12/IL-23 p40 Antibody MAB4991