Mouse IL-1 RI PE-conjugated Antibody Summary
Leu20-Lys338
Accession # P13504
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑1 RI in Mouse CD3+Splenocytes by Flow Cytometry. Mouse CD3+splenocytes were stained with Rat Anti-Mouse IL-1 RI PE-conjugated Monoclonal Antibody (Catalog # FAB7712P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of IL‑1 RI in RAW264 cells by Flow Cytometry. RAW264 cells were stained with Rat Anti-Mouse IL‑1 RI PE‑conjugated Monoclonal Antibody (Catalog # FAB7712P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-1 RI
The type I IL-1 receptor (IL-1 RI, designated IL-1 R1 and CD121a) is one of at least nine members of the IL-1 R family within the Toll/IL-1 R (TIR) superfamily. IL‑1 RI is an ~80 kDa type I transmembrane (TM) protein that binds the pleiotropic cytokines IL-1 alpha and IL-1 beta, plus the IL-1 receptor antagonist (IL-1 Ra). Signal transduction requires complex formation with the IL-1 R accessory protein (IL-1 R AcP/IL-1 R3), another type I TM protein. This complex recruits the adaptor protein MyD88 to initiate signaling in the NF kappa B pathway. Mouse IL-1 RI cDNA encodes a 576 amino acid (aa) protein that contains a 19 aa signal sequence, a 319 aa extracellular domain (ECD) with three C2-type Ig-like domains, a 21 aa TM domain and a 217 aa cytoplasmic region with a TIR domain. Mouse IL-1 RI shares 64%, 83%, 60%, 61% and 55% aa identity with human, rat, canine, equine and bovine IL-1 RI, respectively. The role of IL-1 in inflammation is under several levels of control, including expression and activation of IL-1 alpha and IL-1 beta, expression of IL-1 RI and its accessory and adaptor proteins, and negative regulators such as the IL‑receptor family member, IL-1 RII/IL-1 R2. IL-1 RI is expressed predominantly by T cells, fibroblasts, and endothelial cells and mediates acute phase inflammatory responses including fever.
Product Datasheets
Citations for Mouse IL-1 RI PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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TLR9 Regulates the NF-?B-NLRP3-IL-1? Pathway Negatively in Salmonella-Induced NKG2D-Mediated Intestinal Inflammation
Authors: Y Li, M Liu, Z Zuo, J Liu, X Yu, Y Guan, R Zhan, Q Han, J Zhang, R Zhou, R Sun, Z Tian, C Zhang
J. Immunol., 2017-06-02;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
TLR4-Upregulated IL-1? and IL-1RI Promote Alveolar Macrophage Pyroptosis and Lung Inflammation through an Autocrine Mechanism
Sci Rep, 2016-08-16;6(0):31663.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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