Mouse IFN-gamma R1/CD119 Antibody

Name: Mouse IFN-gamma R1/CD119 Antibody
Brand: R&D Systems
SKU: MAB10261
Rating: 5 (1 reviews)

Catalog #: MAB10261
2 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10261-SP
MAB10261-100
MAB10261-500

All IFN-gamma R1/CD119 Antibodies

Product Details

Citations (2)

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Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IFN-gamma R1/CD119 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IFN‑ gamma R1/CD119 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) IFN-gamma R1, rhIFN-gamma R2, or rhIL-10 R beta is observed.

Source

Monoclonal Hamster IgG Clone # 1F1.93.2

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse IFN‑ gamma R1/CD119
Extracellular domain

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse IFN-gamma R1/CD119 Fc Chimera (Catalog # 1026-GR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma R1/CD119

The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% overall sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN‑ gamma R1 share 52% amino acid sequence identity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN‑ gamma R1 that binds IFN-gamma has been detected in biological fluids.

References

Bach, E.A. et al. (1997) Annu. Rev. Immunol. 15:563.

Long Name

Interferon gamma Receptor 1

Entrez Gene IDs

3459 (Human); 15979 (Mouse)

Alternate Names

AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma R1; IFN-gamma receptor 1; IFNgammaR1; IFN-gamma-R1; IFNGR; IFNGR1; IFN-gR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IFN-gamma R1/CD119 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Retinoid-X-receptors (alpha/beta) in melanocytes modulate innate immune responses and differentially regulate cell survival following UV irradiation.
Authors: Coleman D, Garcia G, Hyter S, Jang H, Chagani S, Liang X, Larue L, Ganguli-Indra G, Indra A
PLoS Genet, 2014-05-08;10(5):e1004321.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes.
Authors: Krejci P, Prochazkova J, Bryja V, Jelinkova P, Pejchalova K, Kozubik A, Thompson LM, Wilcox WR
Cell. Signal., 2008-10-12;21(1):151-60.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot