Mouse Granzyme G Antibody

Catalog # Availability Size / Price Qty
AF1346
AF1346-SP
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Mouse Granzyme G Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Granzyme G in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant mouse Granzyme D is observed and less than 5% cross-reactivity with recombinant human Granzyme H and recombinant mouse Granzyme B is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Granzyme G
Ile21-Leu248
Accession # P13366
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Granzyme G (Catalog # 1346-SE)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Granzyme G (Catalog # 1346-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Granzyme G

Granzyme G is a member of the Granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1). Together with Granzymes D, E and F, it is regulated through pregnancy and by IL-2 and IL-15 in granulated metrial gland cells (2). Human or rat counterpart of mouse Granzyme G has not been found. Like other Granzymes, mouse Granzyme G is not secreted as a zymogen but stored as a fully processed and activated enzyme in the cytoplasmic granules of CTL (3). It is synthesized as a 248 amino acid precursor with a 18 amino acid signal peptide and a 2 amino acid propeptide (3, 4). The mature protein (residues 21‑248) is expressed and purified.

References
  1. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  2. Allen, M.P. and M. Nilsen-Hamilton (1998) J. Immunol. 161:2772.
  3. Jenne, D.E. et al. (1989) Biochemistry 28:7953.
  4. Kwon, B.S. et al. (1988) J. Exp. Med. 168:1839.
Entrez Gene IDs
14944 (Mouse); 266704 (Rat)
Alternate Names
Ctla7; Ctla-7; Granzyme G; GrzG; GZMG

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