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Mouse Granzyme G Antibody

Catalog #: MAB1346 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1346
MAB1346-SP
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Mouse Granzyme G Antibody Summary

Specificity
Detects mouse Granzyme G in direct ELISAs and Western blots. In Western blots, approximately 5% cross‑reactivity with recombinant mouse Granzyme D is observed.
Source
Monoclonal Rat IgG2A Clone # 207639
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Granzyme G
Ile21-Leu248
Accession # P13366
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Granzyme G (Catalog # 1346-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Granzyme G

Granzyme G is a member of the Granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1). Together with Granzymes D, E and F, it is regulated through pregnancy and by IL‑2 and IL‑15 in granulated metrial gland cells (2). A human or rat counterpart of mouse Granzyme G has not been found. Like other Granzymes, mouse Granzyme G is not secreted as a zymogen but stored as a fully processed and activated enzyme in the cytoplasmic granules of CTL (3). It is synthesized as a 248 amino acid (aa) precursor with a 18 aa signal peptide and a 2 aa propeptide (3, 4).

References
  1. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  2. Allen, M.P. and M. Nilsen-Hamilton (1998) J. Immunol. 161:2772.
  3. Jenne, D.E. et al. (1989) Biochemistry 28:7953.
  4. Kwon, B.S. et al. (1988) J. Exp. Med. 168:1839.
Entrez Gene IDs
14944 (Mouse); 266704 (Rat)
Alternate Names
Ctla7; Ctla-7; Granzyme G; GrzG; GZMG

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