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Mouse GLI-2 Antibody

Catalog #: AF3635
18 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3635
AF3635-SP
GLI‑2 in Mouse Embryo.
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Product Details
Citations (18)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse GLI-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GLI-2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse GLI-2
Met1-Glu416
Accession # NP_001074594
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse GLI‑2
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry GLI-2 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

GLI‑2 in Mouse Embryo. GLI-2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse GLI-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3635) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing muscle. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLI-2

Mouse GLI-2 is a 185 kDa biopotential transcription regulator of the hedgehog signaling pathway. It contains 5 conserved tandem C2H2 zinc finger domains that are flanked by a repression domain at the N-terminal region and an activation domain in the C-terminal region. At least two additional isoforms that have amino acid substitutions/truncations in the region corresponding to amino acid residues 192‑350 of isoform 1 have been described. Transcriptional activity of GLI-2 is regulated by proteolytic processing and degradation. Within the region used as immunogen, mouse GLI-2 shares 91% and 97% amino acid sequence homology with that of human and rat GLI-2, respectively.

Long Name
GLI-Kruppel family member GLI2
Entrez Gene IDs
2736 (Human); 14633 (Mouse); 304729 (Rat)
Alternate Names
GLI family zinc finger 2; GLI2; GLI-2; GLI-Kruppel family member GLI2; glioma-associated oncogene family zinc finger 2; HPE9tax helper protein 1; oncogene GLI2; tax helper protein 2; Tax helper protein; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; THP; THP1; THP2; zinc finger protein GLI2

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Citations for Mouse GLI-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal development
    Authors: J Sun, J Yoon, M Lee, HK Lee, YS Hwang, IO Daar
    Cell Reports, 2022-02-01;38(5):110312.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  2. Activation of Cilia-Independent Hedgehog/GLI1 Signaling as a Novel Concept for Neuroblastoma Therapy
    Authors: A Koeniger, A Brichkina, I Nee, L Dempwolff, A Hupfer, I Galperin, F Finkernage, A Nist, T Stiewe, T Adhikary, W Diederich, M Lauth
    Cancers, 2021-04-15;13(8):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. A Shh/Gli-driven three-node timer motif controls temporal identity and fate of neural stem cells
    Authors: JM Dias, Z Alekseenko, A Jeggari, M Boareto, J Vollmer, M Kozhevniko, H Wang, MP Matise, A Alexeyenko, D Iber, J Ericson
    Science Advances, 2020-09-16;6(38):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation, Western Blot
  4. A Smo/Gli Multitarget Hedgehog Pathway Inhibitor Impairs Tumor Growth
    Authors: L Lospinoso, D Quaglio, I Basili, F Ghirga, F Bufalieri, M Caimano, S Balducci, M Moretti, I Romeo, E Loricchio, M Maroder, B Botta, M Mori, P Infante, L Di Marcotu
    Cancers (Basel), 2019-10-09;11(10):.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  5. NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling
    Authors: A Martin-Hur, R Martin-Mor, N Robledinos, R Blanco, I Palacios-B, I Lastres-Be, A Cuadrado, FR Garcia-Gon
    Sci Rep, 2019-09-25;9(1):13896.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  6. Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning
    Authors: P Ma, NN Song, Y Li, Q Zhang, L Zhang, L Zhang, Q Kong, L Ma, X Yang, B Ren, C Li, X Zhao, Y Li, Y Xu, X Gao, YQ Ding, B Mao
    Cell Rep, 2019-07-09;28(2):541-553.e4.
    Species: Human, Mouse
    Sample Types: Cell Fractions, Cell Lysates
    Applications: Western Blot
  7. Itch/?-arrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signalling and medulloblastoma tumorigenesis
    Authors: P Infante, R Faedda, F Bernardi, F Bufalieri, L Lospinoso, R Alfonsi, D Mazzà, M Siler, S Coni, A Po, M Petroni, E Ferretti, M Mori, E De Smaele, G Canettieri, C Capalbo, M Maroder, I Screpanti, M Kool, SM Pfister, D Guardavacc, A Gulino, L Di Marcotu
    Nat Commun, 2018-03-07;9(1):976.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms
    Authors: T Rosengren, LJ Larsen, LB Pedersen, ST Christense, LB Møller
    Cell. Mol. Life Sci., 2018-02-02;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling
    Authors: VJ Guen, TE Chavarria, C Kröger, X Ye, RA Weinberg, JA Lees
    Proc. Natl. Acad. Sci. U.S.A., 2017-11-20;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Suppressor of Fused Restraint of Hedgehog Activity Level is Critical for Osteogenic Proliferation and Differentiation during Calvarial Bone Development
    Authors: J Li, Y Cui, J Xu, Q Wang, X Yang, Y Li, X Zhang, M Qiu, Z Zhang, Z Zhang
    J. Biol. Chem., 2017-08-09;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-?2 nuclear import system
    Authors: Y Han, Y Xiong, X Shi, J Wu, Y Zhao, J Jiang
    PLoS Biol., 2017-08-04;15(8):e2002063.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord
    Authors: H Cai, A Liu
    Dev. Biol., 2017-04-12;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling
    Proc. Natl. Acad. Sci. U.S.A, 2016-12-05;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-?/?1-Independent
    PLoS ONE, 2016-08-31;11(8):e0162033.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Pharmacological GLI2 inhibition prevents myofibroblast cell-cycle progression and reduces kidney fibrosis.
    Authors: Kramann R, Fleig S, Schneider R, Fabian S, DiRocco D, Maarouf O, Wongboonsin J, Ikeda Y, Heckl D, Chang S, Rennke H, Waikar S, Humphreys B
    J Clin Invest, 2015-07-20;125(8):2935-51.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  16. Inversion of Sonic hedgehog action on its canonical pathway by electrical activity.
    Authors: Belgacem Y, Borodinsky L
    Proc Natl Acad Sci U S A, 2015-03-17;112(13):4140-5.
    Species: Xenopus
    Sample Types: Embryo
    Applications: IHC-P, Western Blot
  17. Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators.
    Authors: Liu Y, Tsai I, Morleo M, Oh E, Leitch C, Massa F, Lee B, Parker D, Finley D, Zaghloul N, Franco B, Katsanis N
    J Clin Invest, 2014-04-01;124(5):2059-70.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins.
    Authors: Humke EW, Dorn KV, Milenkovic L, Scott MP, Rohatgi R
    Genes Dev., 2010-04-01;24(7):670-82.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot

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Reviews for Mouse GLI-2 Antibody

Average Rating: 3 (Based on 3 Reviews)

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Mouse GLI-2 Antibody
By Oliver Tkaczyk on 08/02/2020
Application: WB Sample Tested: NIH-3T3 mouse embryonic fibroblast cell line Species: Mouse
Western Blot Mouse GLI-2 Antibody AF3635
Mouse GLI-2 Antibody
By Brygida Baran on 07/22/2020
Application: IP Sample Tested: NIH-3T3 mouse embryonic fibroblast cell line Species: Mouse
Immunoprecipitation Mouse GLI-2 Antibody AF3635
Mouse GLI-2 Antibody
By Anonymous on 07/08/2019
Application: WB Sample Tested: MEF (fibroblast) Species: Mouse

This antibody recognizes many bands, and the major expected bands are not stronger (or maybe much weaker) than those non-specific bands.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***