Mouse Frizzled-1 Biotinylated Antibody

Catalog #: BAF1120 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF1120

All Frizzled-1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Frizzled-1 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Frizzled-1 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant mouse (rm) Frizzled-3, rmFrizzled-4, rmFrizzled-7, and rmFrizzled-8 is observed and less than 1% cross-reactivity with rmFrizzled-6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled‑1
Gln1-His248 (Met122Ile)
Accession # O70421

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Frizzled‑1 Fc Chimera (Catalog # 1120-FZ)

Immunohistochemistry

5-15 µg/mL

Immersion fixed frozen sections of mouse embryo (E13)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-1

The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1). Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway (2). Ten mouse and human Frizzled genes have been identified to date. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane region, and a variable-length C-terminal cytoplasmic domain. The CRD, which comprises 642 amino acid residues and shares 95% identity with the human orthologue in the CRD. Frizzled-1 mRNA has been detected in relatively large amounts in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary, and in fetal lung and kidney (3).

Several Frizzled-dependent signaling pathways exist (2). Their activation depends on the Wnt ligand and the cell context. Members of the low density lipoprotein receptor-related protein (LRP) are co-receptors for the Wnt ligands. LRP5/6 serve as co-receptors in the Wnt/Frizzled canonical pathway that alters gene expression via the stabilization of beta -catenin (4, 5). LRP1 may down regulate Wnt-3a/Frizzled-1 signaling in the canonical pathway by sequestering Frizzled-1 (6). Frizzled-1 is one of the purported Wnt-10b receptors whose signaling inhibits adipogenesis in preadipocytes (7). Frizzled-1 may be part of a feedback mechanism to modulate the effects of BMP-2 in mesenchymal cells since upregulation of its expression by BMP-2 counteracts the effects of BMP-2 and Wnt-3a in inducing the expression of the osteoblast differentiation marker, alkaline phosphatase (8).

References

Wodarz, A. and R. Nusse (1998) Annu. Rev. Cell Dev. Biol. 14:59.
Hsieh, J-C. (2004) Front. Biosci., 9:1333.
Xu, L. et al. (2001) Matrix Biol. 2:147.
Tamai, K. et al. (2000) Nature 407:530.
Pinson, K. et al. (2000) Nature 407:535.
Zielberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
Bennet, C. et al. (2002) J. Biol. Chem. 277:30998.
Roman-Roman, S. et al. (2004) J. Biol. Chem. 279:5725.

Entrez Gene IDs

8321 (Human); 14362 (Mouse)

Alternate Names

DKFZp564G072; FLJ95923; frizzled family receptor 1; frizzled homolog 1; frizzled, Drosophila, homolog of, 1; Frizzled1; Frizzled-1; fz-1; FZD1; fzE1; hFz1; seven transmembrane spanning receptor; Wnt receptor