Mouse FGFR5/FGFRL1 Antibody

Catalog #: MAB1899 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1899
MAB1899-SP

All FGFR5/FGFRL1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse FGFR5/FGFRL1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse FGF R5/FGFRL1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) FGF R1 alpha (IIIb), 1 alpha (IIIc), 1 beta (IIIb), 1 beta (IIIc), recombinant mouse (rm) FGF R2 alpha (IIIb), 2 alpha (IIIc), 2 beta (IIIb), 2 beta (IIIc), 3 alpha (IIIb), 3 alpha (IIIc), rmFGF R4, or rhFGF R5 is observed. Was not tested for cross-reactivity with rmFGF R5 gamma.

Source

Monoclonal Rat IgG_2B Clone # 223003

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse FGF R5/FGFRL1
Ala21-Pro374
Accession # Q91V87

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse FGF R5/FGFRL1 Fc Chimera (Catalog # 1899-FR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR5/FGFRL1

Fibroblast growth factor receptor 5 (FGF R5), also known as FGRL1, is a 65 kDa transmembrane member of the FGF receptor family (1). Mature mouse FGF R5 consists of a 354 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 134 aa cytoplasmic domain (2). FGF R5 is distinct from other FGF receptor family members in that it does not contain a cytoplasmic tyrosine kinase domain (2, 3). Within the ECD, mouse FGF R5 shares 94% and 98% aa sequence identity with human and rat FGF R5, respectively. Alternate splicing results in an isoform that lacks the first Ig-like domain (2). FGF R5 is widely expressed, and expression of two species of mRNA in cartilage and pancreas may indicate the presence of both splice forms (2‑6). Both the full length and truncated isoforms of FGF R5 bind FGF basic; the full length form additionally binds to heparin (2, 6). FGF R5 may function as a decoy receptor by binding FGF but not transducing its mitogenic signals (6).

References

Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
Sleeman, M. et al. (2001) Gene 271:171.
Kim, I. et al. (2001) Biochim. Biophys. Acta 1518:152.
Antoine, M. et al. (2005) Growth Factors 23:87.
Wiedemann, M. et al. (2000) Genomics 69:275.
Trueb, B. et al. (2003) J. Biol. Chem. 278:33857.

Long Name

Fibroblast Growth Factor Receptor-like 1

Entrez Gene IDs

53834 (Human); 116701 (Mouse)

Alternate Names

FGF homologous factor receptor; FGF R5; FGF receptor-like protein 1; FGFR5; FGFR-5; FGFRL1; FGFR-like protein; FHFR; Fibroblast growth factor receptor 5; fibroblast growth factor receptor-like 1