Mouse Fc gamma RII/RIII (CD32/CD16) Biotinylated Antibody

Catalog #: BAF1460 Datasheet / COA / SDS

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BAF1460

All Fc gamma RII/RIII (CD32/CD16) Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Fc gamma RII/RIII (CD32/CD16) Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Fc gamma RII/RIII (CD32/CD16) in ELISAs and Western blots. In sandwich immunoassays, less than 10% cross-reactivity with recombinant mouse CD16 is observed and less than 0.1% cross-reactivity with recombinant human CD32 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIIB
Thr40-Arg217
Accession # NP_034317

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Fc gamma RIIB/CD32b (Catalog # 1460-CD)

Mouse Fc gamma RIIA/CD32a Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse Fc gamma RIIB/CD32b Antibody (Catalog # MAB14601)

Standard: Recombinant Mouse Fc gamma RIIB/CD32b Protein, CF (Catalog # 1460-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RII/RIII (CD32/CD16)

Fc gamma RII, also named CD32, is a low-affinity receptor for the Fc region of complexed IgG. Three genes for human Fc gamma RII (A, B and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins, have been identified. The extracellular domain of mouse Fc gamma RII shares approximately 95% amino acid sequence identity with that of mouse Fc gamma RIII/CD16.

Long Name

Fc gamma Receptor II/III

Alternate Names

CD16;CD16A;CD16-II;FCG3;FCGR3;FCGR3A;FCGRIII;FcGRIIIA;FCR-10;FCRIII;FCRIIIA;IGFR3;IMD20;Low affinity immunoglobulin gamma Fc region receptor III-A; CD32/CD16; Fc gamma RII/RIII