Home / Fc gamma RI/CD64 / Mouse Fc gamma  RI/CD64 PE-conjugated Antibody

Mouse Fc gamma  RI/CD64 PE-conjugated Antibody

Catalog #: FAB20741P
4 Citations Datasheet / COA / SDS
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FAB20741P
Detection of Fc gamma RI/CD64 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Citations (4)
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Mouse Fc gamma  RI/CD64 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Fc gamma  RI/CD64 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Fc gamma  RIA, rhFc gamma  RIIB, or rhFc gamma  RIIIB is observed.
Source
Monoclonal Rat IgG2A Clone # 290322
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0-derived recombinant mouse Fc gamma RI/CD64 extracellular domain
Glu25-Pro297
Accession # P26151
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Fc? RI/CD64 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Fc gamma RI/CD64 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Fc gamma RI/CD64 PE-conjugated Monoclonal Antibody (Catalog # FAB20741P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Fc gamma RI/CD64

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1-3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10‑8-10‑9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10‑6-10‑7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5). Mouse Fc gamma RI is transmembrane protein with three extracellular Ig-like domains, and it delivers an activating signal via the associated Fc R gamma accessory chain (1, 2). The high affinity recognition of IgG by Fc gamma RI permits the triggering of effector responses at low IgG concentrations typical of early immune responses (2). Fc gamma RI is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils (1, 4). Its expression is up-regulated during bacterial infections and sepsis.

References
  1. Van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.
Long Name
Fc gamma Receptor I
Entrez Gene IDs
2209 (Human); 14129 (Mouse); 295279 (Rat); 102147198 (Cynomolgus Monkey)
Alternate Names
CD64 antigen; CD64; CD64a; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc gamma RI; FCG1; Fc-gamma receptor I B2; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FCGR1; FcgRI; FcgRIA; FCRI; FcRIA; FLJ18345; high affinity Ia, receptor for (CD64); high affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I

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Citations for Mouse Fc gamma  RI/CD64 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Aging-Impaired Filamentous Actin Polymerization Signaling Reduces Alveolar Macrophage Phagocytosis of Bacteria
    Authors: Z Li, Y Jiao, EK Fan, MJ Scott, Y Li, S Li, TR Billiar, MA Wilson, X Shi, J Fan
    J. Immunol., 2017-09-25;0(0):.
  2. The 5-Phosphatase SHIP2 Promotes Neutrophil Chemotaxis and Recruitment
    Authors: Melina Michael, Barry McCormick, Karen E. Anderson, Utsa Karmakar, Matthieu Vermeren, Stéphane Schurmans et al.
    Frontiers in Immunology
  3. TREM2 deficiency reduces the efficacy of immunotherapeutic amyloid clearance
    EMBO Mol Med, 2016-09-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. PKC-epsilon pseudosubstrate and catalytic activity are necessary for membrane delivery during IgG-mediated phagocytosis.
    Authors: Wood T, Chow R, Hanes C, Zhang X, Kashiwagi K, Shirai Y, Trebak M, Loegering D, Saito N, Lennartz M
    J Leukoc Biol, 2013-05-13;94(1):109-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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