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Mouse FABP5/E-FABP Biotinylated Antibody

Catalog #: BAF1476
6 Citations Datasheet / COA / SDS
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BAF1476
Product Details
Citations (6)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse FABP5/E-FABP Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse FABP5/E‑FABP in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human FABP3 is observed and less than 1% cross-reactivity with recombinant mouse FABP4, recombinant rat (rr) FABP1 and rrFABP2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse FABP5/E‑FABP
Met1-Gln135
Accession # Q05816
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse FABP5
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse intestine, liver, skin, and thymus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP5/E-FABP

Mouse FABP5, also named Keratinocyte lipid-binding protein (KLBP) and Mal-1, is a member of the intracellular FABP family. It is expressed most abundantly in keratinocytes but is also found in differentiated adipocytes, macrophages and stratified epithelia of epidermis and tongue.

Long Name
Fatty Acid-Binding Protein 5
Entrez Gene IDs
2171 (Human); 16592 (Mouse); 140868 (Rat)
Alternate Names
EFABP; E-FABP; E-FABPPAFABP; FABP5; fatty acid binding protein 5 (psoriasis-associated); Fatty acid-binding protein 5; PA-FABPepidermal; Psoriasis-associated fatty acid-binding protein homolog

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Citations for Mouse FABP5/E-FABP Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Transgelin 2 guards T cell lipid metabolic programming and anti-tumor function
    Authors: Hwang, SM;Awasthi, D;Jeong, J;Sandoval, TA;Chae, CS;Ramos, Y;Tan, C;Falco, MM;McBain, IT;Mishra, B;Ivashkiv, LB;Zamarin, D;Cantillo, E;Chapman-Davis, E;Holcomb, K;Morales, DK;Rodriguez, PC;Conejo-Garcia, JR;Kaczocha, M;Vähärautio, A;Song, M;Cubillos-Ruiz, JR;
    Research square
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. Glutamate transporter Slc1a3 mediates inter-niche stem cell activation during skin growth
    Authors: B Reichenbac, J Classon, T Aida, K Tanaka, M Genander, C Göritz
    EMBO J., 2018-04-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. WNT10A mutation causes ectodermal dysplasia by impairing progenitor cell proliferation and KLF4-mediated differentiation
    Authors: M Xu, J Horrell, M Snitow, J Cui, H Gochnauer, CM Syrett, S Kallish, JT Seykora, F Liu, D Gaillard, JP Katz, KH Kaestner, B Levin, C Mansfield, JE Douglas, BJ Cowart, M Tordoff, F Liu, X Zhu, LA Barlow, AI Rubin, JA McGrath, EE Morrisey, EY Chu, SE Millar
    Nat Commun, 2017-06-07;8(0):15397.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. The histone methyltransferase Setd8 acts in concert with c-Myc and is required to maintain skin.
    Authors: Driskell I, Oda H, Blanco S
    EMBO J., 2011-11-25;31(3):616-29.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Dose and context dependent effects of Myc on epidermal stem cell proliferation and differentiation
    Authors: Melanie A. Berta, Christopher M. Baker, Denny L. Cottle, Fiona M. Watt
    EMBO Molecular Medicine
  6. Dynamic regulation of retinoic acid-binding proteins in developing, adult and neoplastic skin reveals roles for beta-catenin and Notch signalling.
    Authors: Collins CA, Watt FM
    Dev. Biol., 2008-09-12;324(1):55-67.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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