Mouse Endosialin/CD248 Antibody

Catalog #: MAB7535 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7535
MAB7535-SP

Endosialin/CD248 in bEnd.3 Mouse Cell Line.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Endosialin/CD248 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Endosialin/CD248 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human Endosialin/CD248 is observed.

Source

Monoclonal Rat IgG_2A Clone # 458606

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Endosialin/CD248
Val13-Arg693 (predicted)
Accession # Q91V98

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Endosialin/CD248 in bEnd.3 Mouse Cell Line. Endosialin/CD248 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse Endosialin/CD248 Monoclonal Antibody (Catalog # MAB7535) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry

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Endosialin/CD248 in IMR-32 Human Cell Line. Endosialin/CD248 was detected in immersion fixed IMR-32 human neuroblastoma cell line using Rat Anti-Mouse Endosialin/CD248 Monoclonal Antibody (Catalog # MAB7535) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endosialin/CD248

Endosialin, also known as CD248 and tumor endothelial marker 1 (Tem1), is a 165 kDa transmembrane O-glycosylated protein that contains one C-type lectin, one Sushi, one EGF-like domain and a mucin-like stalk in its extracellular domain (ECD). It is expressed on activated perivascular and stromal cells in embyronic and tumor neovasculature but is downregulated in quiescent vasculature. Endosialin regulates pericyte proliferation, migration, and adhesion to matrix Fibronectin and Collagens I and IV. It is also required for lymph node and spleen remodeling during immune responses. Within the ECD, mouse Endosialin shares 76% and 93% aa sequence identity with human and rat Endosialin, respectively.

Entrez Gene IDs

57124 (Human); 70445 (Mouse); 293669 (Rat)

Alternate Names

2610111G01Rik; CD164 sialomucin-like 1; CD164L1; CD248 antigen; CD248 antigen, endosialin; CD248 molecule, endosialin; CD248; Endosialin; MGC119479; TEM1; TEM1MGC119478; Tumor endothelial marker 1