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Mouse EBI3 PE-conjugated Antibody

Catalog #: IC18341P
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC18341P
Detection of IL-27/IL-35 EBI3 Subunit in Mouse splenocytes by Flow Cytometry.
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Citations (3)
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Mouse EBI3 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-27/IL-35 EBI3 Subunit in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human IL‑27/IL-35 EBI3 subunit.
Source
Monoclonal Rat IgG2A Clone # 355022
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑27 heterodimer
Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28)
Accession # O35228 (EBI3) & Q8K3I6 (p28)
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-27/IL-35 EBI3 Subunit antibody in Mouse splenocytes antibody by Flow Cytometry. View Larger

Detection of IL-27/IL-35 EBI3 Subunit in Mouse splenocytes by Flow Cytometry. Mouse splenocytes untreated (open histogram) or treated with LPS (filled histogram) were stained with Rat Anti-Mouse IL-27/IL-35 EBI3 Subunit PE-conjugated Monoclonal Antibody (Catalog # IC18341A) or isotype control antibody (Catalog # IC006P, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: EBI3

IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide anda 209 aa mature protein with two fibronectin type III domains.

Long Name
Epstein-Barr virus induced gene 3
Entrez Gene IDs
10148 (Human); 50498 (Mouse); 680609 (Rat); 102144621 (Cynomolgus Monkey)
Alternate Names
cytokine receptor; EBI3; EBI-3; EBV-induced gene 3 protein; Epstein-Barr virus induced 3; Epstein-Barr virus induced gene 3; Epstein-Barr virus-induced gene 3 protein; IL-27 beta; IL-27 subunit beta; IL27B; IL-27B; IL35 EBI3; IL-35 EBI3; interleukin-27 subunit beta

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Citations for Mouse EBI3 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Balance between immunoregulatory B cells and plasma cells drives pancreatic tumor immunity
    Authors: Bhalchandra Mirlekar, Yan Wang, Sirui Li, Mi Zhou, Sarah Entwistle, Tristan De Buysscher et al.
    Cell Reports Medicine
  2. Human placental trophoblast cells contribute to maternal-fetal tolerance through expressing IL-35 and mediating iTR35 conversion
    Authors: J Liu, S Hao, X Chen, H Zhao, L Du, H Ren, C Wang, H Mao
    Nat Commun, 2019-10-10;10(1):4601.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Regulatory T cells and their derived cytokine, interleukin-35, reduce pain in experimental autoimmune encephalomyelitis
    Authors: SS Duffy, BA Keating, CJ Perera, JG Lees, RS Tonkin, PGS Makker, P Carrive, O Butovsky, G Moalem-Tay
    J. Neurosci., 2019-01-16;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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