Mouse DPPIV/CD26 Biotinylated Antibody

Catalog #: BAF954 Datasheet / COA / SDS

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BAF954

Detection of DPPIV/CD26 in Mouse Thymus.

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Mouse DPPIV/CD26 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse DPPIV/CD26 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human DPPIV/CD26 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse DPPIV/CD26
Ser29-His760
Accession # P28843

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse DPPIV/CD26 (Catalog # 954-SE)

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of mouse thymus

Mouse DPPIV/CD26 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse DPPIV/CD26 Antibody (Catalog # MAB954)

Standard: Recombinant Mouse DPPIV/CD26 Protein, CF (Catalog # 954-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of DPPIV/CD26 in Mouse Thymus. DPPIV/CD26 was detected in immersion fixed paraffin-embedded sections of mouse thymus using Goat Anti-Mouse DPPIV/CD26 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF954) at 10 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPIV/CD26

DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (1, 2). It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain (3‑5). The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression.

DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a co‑factor for entry of HIV in CD4⁺ cells (6). It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans (7). It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine (8, 9). It degrades peptide hormones such as glucagon (10). It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis (11).

References

Misumi, Y. and Y. Ikehara (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (eds), p. 1905, Elsevier, London.
Ikehara, Y. et al. (1994) Methods Enzymol. 244:215.
Marguet, D. et al. (1992) J. Biol. Chem. 267:2200.
Bernard, A.M. et al. (1994) Biochemistry 33:15204.
Vivier, I. et al. (1991) J. Immunol. 147:447.
Callebaut, C. et al. (1993) Science 262:2045.
Kameoka, J. et al. (1993) Science 261:466.
Ohtsuki, T. et al. (1998) FEBS Lett. 431:236.
Proost, P. et al. (1999) J. Biol. Chem. 274:3988.
Hinke, S.A. et al. (2000) J. Biol. Chem. 275:3827.
Wrenger, S. et al. (2000) FEBS Lett. 466:155.

Long Name

Dipeptidyl-peptidase IV

Entrez Gene IDs

1803 (Human); 13482 (Mouse); 397492 (Porcine); 102133935 (Cynomolgus Monkey)

Alternate Names

ADABP; ADCP-2; ADCP2DPP IV; Adenosine deaminase complexing protein 2TP103; CD26 antigen; CD26; CD26T-cell activation antigen CD26; dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidyl-peptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4; DPPIV; EC 3.4.14.5