Mouse DPPA5/ESG1 Antibody

Catalog #: AF3984 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3984
AF3984-SP

All DPPA5/ESG1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse DPPA5/ESG1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse DPPA5/ESG1 in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human DPPA5 is observed and approximately 10% cross-reactivity with recombinant mouse DPPA4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse DPPA5/ESG1
Met1-Glu118
Accession # Q9CQS7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse DPPA5/ESG1

Immunocytochemistry

5-15 µg/mL

Immersion fixed D3 mouse embryonic stem cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPA5/ESG1

DPPA5, also known as Esg-1 and ECAT2, is a 14 kDa RNA-binding protein expressed in early embryos, primordial germ cells, and embryonic stem cells and is used as a marker of the pluripotent state. Mouse DPPA5 is 118 amino acids in length and shows 63% amino acid identity with human and 81% amino acid identity with rat DPPA5 proteins.

Long Name

Developmental Pluripotency Associated 5

Entrez Gene IDs

340168 (Human); 434423 (Mouse); 301101 (Rat)

Alternate Names

developmental pluripotency associated 5; developmental pluripotency-associated 5 protein; DPPA5; embryonal stem cell specific gene 1; Embryonal stem cell-specific gene 1 protein; ESG1; ESG1ESG-1; hDPPA5