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Mouse Dkk-4 Antibody

Catalog #: AF3105
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3105
AF3105-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Dkk-4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Dkk-4 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Dkk-4, recombinant mouse (rm) Dkk-1, rmDkk-2, and rmDkk-3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Dkk-4
Leu19-Ile221 (Arg67Ser, Arg70Ser)
Accession # Q8VEJ3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Dkk‑4 (Catalog # 3105-DK)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dkk-4

Dickkopf related protein 4 (Dkk-4) is a member of the Dickkopf protein family that includes Dkk-1, -2, -3, and -4 and a related protein, Soggy (1). Expression of Dkk-4 has only been shown with sensitive PCR techniques during early embryonic development in mice (2), in differentiated human ES cells (3), or in mice that express dominant-active beta -catenin elevating Wnt signaling in the forebrain (4). Dkk proteins are secreted proteins that are synthesized as precursors with an N-terminal signal peptide; all have two conserved cysteine-rich domains separated by a linker region which contains a potential furin type proteolytic cleavage site. The domains contain 10 cysteines each; prokineticin and colipase families show a configuration of cysteines similar to the second motif (5). Mouse Dkk-4 shows 91%, 72% and 71% amino acid (aa) identity with rat, human and canine Dkk-4, respectively, and 40‑46% aa identity with other mouse Dkk proteins. Dkk-4 is predicted as a 25 kDa protein, but transfection of 293T cells produces a shorter (15‑17 kDa) form containing only the second cysteine-rich domain, as well as longer (30‑32 and 40 kDa) forms that do not appear to be glycosylated or form covalent multimers (1). Of the four Dkk proteins, Dkk-4 is the most like Dkk-1. Both are unequivocal antagonists of the canonical Wnt signaling pathway (1, 6), which is activated by Wnt protein engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6 (7). Dkk-1 and Dkk-4 antagonize Wnt by direct high-affinity binding to LRP5/6, forming ternary complexes of LRP5/6 with the Kremens protein Krm2. Internalization of the complex is triggered, making LRP5/6 unavailable for interaction with Wnt ligands (6‑9).

References
  1. Krupnik, V.E. et al. (1999) Gene 238:301.
  2. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  3. Katoh, Y. and M. Katoh (2005) Int. J. Mol. Med. 16:477.
  4. Diep, D.B. et al. (2004) Dev. Brain Res. 153:261.
  5. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582.
  6. Mao, B. and C. Niehrs (2003) Gene 302:179.
  7. Logan, C.Y. and R. Nusse (2004) Ann. Rev. Cell Dev. Biol. 20:781.
  8. Mao, B. et al. (2002) Nature 417:664.
  9. Mao, B. et al. (2001) Nature 411:321.
Long Name
Dickkopf-4
Entrez Gene IDs
27121 (Human); 234130 (Mouse)
Alternate Names
dickkopf (Xenopus laevis) homolog 4; dickkopf homolog 4 (Xenopus laevis); dickkopf-4; dickkopf-related protein 4; Dkk4; Dkk-4; hDkk-4; MGC129562; MGC129563

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Citations for Mouse Dkk-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Molecular dynamics of Dkk4 modulates Wnt action and regulates Meibomian gland development
    Authors: David Schlessing
    Development, 2016-11-18;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. The effects of Dickkopf-4 on the proliferation, differentiation, and apoptosis of osteoblasts.
    Authors: Hiramitsu S, Terauchi M, Kubota T
    Endocrinology, 2013-10-08;154(12):4618-26.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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