Mouse Dectin-1/CLEC7A Antibody Summary
Phe69-Leu244
Accession # Q6QLQ4
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dectin-1/CLEC7A
Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin‑1, respectively. It shares 25% - 34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8 ‑ 10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11 ‑ 13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).
- Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
- Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
- Ariizumi, K. et al. (2000) J. Biol. Chem. 275:20157.
- Heinsbroek, S.E.M. et al. (2006) J. Immunol. 176:5513.
- Taylor, P.R. et al. (2002) J. Immunol. 169:3876.
- Willment, J.A. et al. (2003) J. Immunol. 171:4569.
- Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
- Gantner, B.N. et al. (2005) EMBO J. 24:1277.
- Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
- Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
- Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
- Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
- Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
- Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
- Saijo, S. et al. (2007) Nat. Immunol. 8:39.
- Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Product Datasheets
Citation for Mouse Dectin-1/CLEC7A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Protein-tyrosine phosphatase Shp2 positively regulates macrophage oxidative burst.
Authors: Li X, Goodwin C, Nabinger S, Richine B, Yang Z, Hanenberg H, Ohnishi H, Matozaki T, Feng G, Chan R
J Biol Chem, 2014-12-23;290(7):3894-909.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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Average Rating: 2.5 (Based on 2 Reviews)
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HEK cells were transfected with mouse Dectin1 isoform 2 and stained with the Rat anti-mouse Dectin1 clone 218838 at a concentration of 0.005 nM to 300 nM. The 218838 antibody was detected using a secondary DyLight 405 AffiniPure Mouse Anti-Rat IgG, F(ab')₂ fragment specific antibody. The binding affinity was calculated at EC50 = 64 nM. Binding was observed only in the higher concentrations of the antibody.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
***UPDATE:
Thank you so much for taking the time to share your experience using RnD Systems Antibodies. We value your opinion and use the online reviews as testimonials from our customers, of the quality of our products. Our technical service representatives work hard to resolve any issues with our products when customers report they have not had success.
The immunogen used for MAB1756 is Mouse myeloma cell line NS0-derived recombinant mouse Dectin-1 (Phe69-Leu244, Accession # Q6QLQ4), which is Dectin1 isoform 1. Dectin1 isoform 2 was transfected and used in customer’s assay, which misses aa69-aa113 compared to isoform 1. We have not done epitope mapping for MAB1756 and therefore we don’t know which part of Dectin1 isoform 1 this antibody binds. We have not tested whether MAB1756 is able to detect isoform 2.
Tech Service tried to reach the customer and didn’t receive a response. We would appreciate if you would be willing to contact a technical service representative at techsupport@bio-techne.com and allow us to troubleshoot the results you observed with MAB1756, that you reported in your online review. We look forward to hearing from you.***
Detection of stalkless mouse Dectin1 isoform B in transfected HEK cells by Flow Cytometry. HEK cells overexpressing the murine Dectin1B isoform (stalkless) were stained with the Rat anti-mouse
Dectin1 clone 218838 at 100 nM or the Rat IgG2B isotype control. Binding was detected with a secondary a-Rat IgG (H+L) Pacific blue antibody.
