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Mouse CXCR4 APC-conjugated Antibody

Catalog #: FAB21651A
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB21651A-100UG
FAB21651A-025UG
Detection of CXCR4 in Mouse Thymocytes by Flow Cytometry.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCR4 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCR4 transfectants. Does not stain irrelevant transfectants.
Source
Monoclonal Rat IgG2B Clone # 247506
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with mouse CXCR4
Met1-Ser359
Accession # P70658
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR4 antibody in Mouse Thymocytes antibody by Flow Cytometry. View Larger

Detection of CXCR4 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with (A) Rat Anti-Mouse CXCR4 APC-conjugated Monoclonal Antibody (Catalog # FAB21651A) or (B) Rat IgG2Bisotype control antibody (Catalog # IC013A) and Rat anti-Mouse CD8 alpha PE-conjugated Monoclonal Antibody (Catalog # FAB116P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR4

CXCR4, also known as CD184, is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).

References
  1. Orsini, M.J. et al. (1999) J. Biol. Chem. 274:31076.
  2. Zagzag, D. et al. (2005) Cancer Res. 65:6178.
  3. Speetjens, F.M. et al. (2009) Cancer Microenvironment 2:1.
  4. Wang, L. et al. (2009) Oncology Reports 22:1333.
  5. Amara, S. et al. (2015) Cancer Biomark. 15:869.
Long Name
C-X-C Motif Chemokine Receptor 4
Entrez Gene IDs
7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)
Alternate Names
CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXCR4; CXC-R4; CXCR-4; D2S201E; D2S201ESDF-1 receptor; FB22; Fusin; HM89; HM89NPYRL; HSY3RR; LAP3; LAP-3; LCR1; LESTR; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3R; NPY3RWHIM; NPYR; NPYRL; NPYY3R; pCXCR4; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor; WHIMS

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Citations for Mouse CXCR4 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Genetic reporter analysis reveals an expandable reservoir of OCT4+ cells in adult skin
    Authors: Anne Limbourg, Sabine Schnabel, Vladimir J Lozanovski, L Christian Napp, Teng-Cheong Ha, Tobias Maetzig et al.
    Cell Regeneration
  2. Osteoblasts protect AML cells from SDF-1-induced apoptosis.
    Authors: Kremer K, Dudakovic A, McGee-Lawrence M, Philips R, Hess A, Smith B, van Wijnen A, Karp J, Kaufmann S, Westendorf J, Hedin K
    J Cell Biochem, 2014-06-01;115(6):1128-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Application of the chemokine CXCL12 expression plasmid restores wound healing to near normal in a diabetic mouse model.
    Authors: Restivo TE, Mace KA, Harken AH, Young DM
    J Trauma, 2010-08-01;69(2):392-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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