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Mouse CXCR2/IL-8RB Antibody

Catalog #: MAB2164
44 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2164-100
MAB2164-SP
MAB2164-500
Detection of CXCR2/IL‑8 RB in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP by Flow Cytometry.
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Citations (44)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCR2/IL-8RB Antibody Summary

Species Reactivity
Mouse
Specificity
Stains mouse CXCR2 transfected cells but not CXCR1 transfectants.
Source
Monoclonal Rat IgG2A Clone # 242216
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
C6 rat glioma cell line transfected with mouse CXCR2
Met1-Leu359
Accession # P35343
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
See below
CyTOF-reported
Wang, G. et al. (2016) Cancer Discov. 6: 80. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize CXCL2/GRO beta /MIP‑2/CINC‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR2. The Neutralization Dose (ND50) is typically 15-50 µg/mL in the presence of 2 ng/mL Recombinant Mouse CXCL2/GRO beta /MIP‑2/CINC‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR2/IL-8 RB antibody in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP antibody by Flow Cytometry. View Larger

Detection of CXCR2/IL‑8 RB in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouse CXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006).

Immunohistochemistry CXCR2/IL-8 RB antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr). View Larger

CXCR2/IL‑8 RB in Mouse Spleen. CXCR2/IL-8 RB was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell sufaces in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Neutralization Chemotaxis Induced by CXCL2/MIP‑2 and Neutralization by Mouse CXCR2/IL‑8 RB Antibody. View Larger

Chemotaxis Induced by CXCL2/MIP‑2 and Neutralization by Mouse CXCR2/IL‑8 RB Antibody. Recombinant Mouse CXCL2/MIP-2 (Catalog # 452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164). The ND50 is typically 15-50 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR2/IL-8RB

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.

Long Name
Interleukin 8 Receptor B
Entrez Gene IDs
3579 (Human); 12765 (Mouse); 29385 (Rat)
Alternate Names
CD182 antigen; CD182; CDw128b; chemokine (C-X-C motif) receptor 2; chemokine (CXC) receptor 2; CMKAR2; C-X-C chemokine receptor type 2; CXCR2 gene for IL8 receptor type B; CXCR2; CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8 RB; IL-8 receptor type 2; IL-8R B; IL8R2; IL8RA; IL8RB; interleukin 8 receptor B; interleukin 8 receptor type 2; interleukin-8 receptor type B

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Citations for Mouse CXCR2/IL-8RB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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  1. Chemokine Cxcl1-Cxcl2 heterodimer is a potent neutrophil chemoattractant
    Authors: Sawant, KV;Sepuru, KM;Penaranda, B;Lowry, E;Garofalo, RP;Rajarathnam, K;
    Journal of leukocyte biology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling
    Authors: S Luo, C Liao, L Zhang, C Ling, X Zhang, P Xie, G Su, Z Chen, L Zhang, T Lai, J Tang
    Cell Reports, 2023-03-14;42(3):112259.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. Interleukin-22 regulates neutrophil recruitment in ulcerative colitis and is associated with resistance to ustekinumab therapy
    Authors: P Pavlidis, A Tsakmaki, E Pantazi, K Li, D Cozzetto, J Digby-Bell, F Yang, JW Lo, E Alberts, ACC Sa, U Niazi, J Friedman, AK Long, Y Ding, CD Carey, C Lamb, M Saqi, M Madgwick, L Gul, A Treveil, T Korcsmaros, TT Macdonald, GM Lord, G Bewick, N Powell
    Nature Communications, 2022-10-03;13(1):5820.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Increased cytokine expression in the choroid plexus stroma and epithelium in response to endotoxin-induced systemic inflammation in mice
    Authors: A Shimada, S Hasegawa-I
    Toxicology reports, 2021-03-10;8(0):520-528.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Frontline Science:�Conversion of neutrophils into atypical Ly6G+ SiglecF+ immune cells with neurosupportive potential in olfactory neuroepithelium
    Authors: K Ogawa, K Asano, S Yotsumoto, T Yamane, M Arita, Y Hayashi, H Harada, C Makino-Oka, H Fukuyama, K Kondo, T Yamasoba, M Tanaka
    J. Leukoc. Biol., 2020-07-29;0(0):.
    Species: Mouse
    Sample Types: Primary whole cells
    Applications: Flow Cytometry
  6. Host syndecan-1 promotes listeriosis by inhibiting intravascular neutrophil extracellular traps
    Authors: RS Aquino, A Hayashida, PW Park
    PLoS Pathog., 2020-05-26;16(5):e1008497.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. Glucocorticoids Promote the Onset of Acute Experimental Colitis and Cancer by Upregulating mTOR Signaling in Intestinal Epithelial Cells
    Authors: Z Zhang, L Dong, A Jia, X Chen, Q Yang, Y Wang, Y Wang, R Liu, Y Cao, Y He, Y Bi, G Liu
    Cancers (Basel), 2020-04-11;12(4):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Novel CD11b+Gr-1+Sca-1+ myeloid cells drive mortality in bacterial infection
    Authors: MY Park, HS Kim, HY Lee, BA Zabel, YS Bae
    Sci Adv, 2020-01-22;6(4):eaax8820.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. The YIN and YANG of lipoproteins in developing and preventing infectious arthritis by Staphylococcus aureus
    Authors: M Mohammad, MT Nguyen, C Engdahl, M Na, A Jarneborn, Z Hu, A Karlsson, R Pullerits, A Ali, F Götz, T Jin
    PLoS Pathog., 2019-06-21;15(6):e1007877.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling
    Authors: ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer
    J. Clin. Invest., 2019-05-20;130(0):2555-2570.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  11. KRAS-IRF2 Axis Drives Immune Suppression and Immune Therapy Resistance in Colorectal Cancer
    Authors: Wenting Liao, Michael J. Overman, Adam T. Boutin, Xiaoying Shang, Di Zhao, Prasenjit Dey et al.
    Cancer Cell
  12. Estrogen stimulates female cancer progression by inducing myeloid-derived suppressive cells: investigations on pregnant and non-pregnant experimental models
    Authors: K Kozasa, S Mabuchi, Y Matsumoto, H Kuroda, E Yokoi, N Komura, M Kawano, R Takahashi, T Sasano, K Shimura, M Kodama, K Hashimoto, K Sawada, K Nagasaka, T Kimura
    Oncotarget, 2019-03-08;10(20):1887-1902.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Cellular content plays a crucial role in Non-typeable Haemophilus influenzae infection of preinflamed Junbo mouse middle ear
    Authors: Pratik P. Vikhe, Tom Purnell, Steve D.M. Brown, Derek W. Hood
    Cellular Microbiology
  14. Distinct Compartmentalization of the Chemokines CXCL1 and CXCL2 and the Atypical Receptor ACKR1 Determine Discrete Stages of Neutrophil Diapedesis
    Authors: Tamara Girbl, Tchern Lenn, Lorena Perez, Loïc Rolas, Anna Barkaway, Aude Thiriot et al.
    Immunity
  15. Novel adherent CD11b+Gr-1+tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment
    Authors: T Tsubaki, T Kadonosono, S Sakurai, T Shiozawa, T Goto, S Sakai, T Kuchimaru, T Sakamoto, H Watanabe, G Kondoh, S Kizaka-Kon
    Oncotarget, 2018-01-29;9(13):11209-11226.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Microbiome-mediated neutrophil recruitment via CXCR2 and protection from amebic colitis
    Authors: K Watanabe, CA Gilchrist, MJ Uddin, SL Burgess, MM Abhyankar, SN Moonah, Z Noor, JR Donowitz, BN Schneider, T Arju, E Ahmed, M Kabir, M Alam, R Haque, P Pramoonjag, B Mehrad, WA Petri
    PLoS Pathog., 2017-08-17;13(8):e1006513.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  17. The mTOR signal regulates myeloid-derived suppressor cells differentiation and immunosuppressive function in acute kidney injury
    Authors: C Zhang, S Wang, J Li, W Zhang, L Zheng, C Yang, T Zhu, R Rong
    Cell Death Dis, 2017-03-23;8(3):e2695.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy.
    Authors: Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce J, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K
    J Biol Chem, 2014-12-11;290(4):2053-68.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. The calcineurin-NFAT axis controls allograft immunity in myeloid-derived suppressor cells through reprogramming T cell differentiation.
    Authors: Wang X, Bi Y, Xue L, Liao J, Chen X, Lu Y, Zhang Z, Wang J, Liu H, Yang H, Liu G
    Mol Cell Biol, 2014-12-01;35(3):598-609.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  20. Platelets guide the formation of early metastatic niches.
    Authors: Labelle, Myriam, Begum, Shahinoo, Hynes, Richard
    Proc Natl Acad Sci U S A, 2014-07-14;111(30):E3053-61.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  21. Disruption of CXCR2-Mediated MDSC Tumor Trafficking Enhances Anti-PD1 Efficacy
    Authors: Steven L. Highfill, Yongzhi Cui, Amber J. Giles, Jillian P. Smith, Hua Zhang, Elizabeth Morse et al.
    Science Translational Medicine
  22. Fibrates ameliorate the course of bacterial sepsis by promoting neutrophil recruitment via CXCR2.
    Authors: Tancevski I, Nairz M, Duwensee K, Auer K, Schroll A, Heim C, Feistritzer C, Hoefer J, Gerner R, Moschen A, Heller I, Pallweber P, Li X, Theurl M, Demetz E, Wolf A, Wolf D, Eller P, Ritsch A, Weiss G
    EMBO Mol Med, 2014-04-06;6(6):810-20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. mTOR limits the recruitment of CD11b+Gr1+Ly6Chigh myeloid-derived suppressor cells in protecting against murine immunological hepatic injury.
    Authors: Zhang Y, Bi Y, Yang H, Chen X, Liu H, Lu Y, Zhang Z, Liao J, Yang S, Chu Y, Yang R, Liu G
    J Leukoc Biol, 2014-02-25;95(6):961-70.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  24. Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments.
    Authors: Sprague L, Muccioli M, Pate M, Singh M, Xiong C, Ostermann A, Niese B, Li Y, Li Y, Courreges M, Benencia F
    Exp Cell Res, 2014-02-22;323(1):7-27.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  25. Phosphatase Wip1 negatively regulates neutrophil migration and inflammation.
    Authors: Sun B, Hu X, Liu G, Ma B, Xu Y, Yang T, Shi J, Yang F, Li H, Zhang L, Zhao Y
    J Immunol, 2014-01-06;192(3):1184-95.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. IL-17A produced by neutrophils protects against pneumonic plague through orchestrating IFN-gamma-activated macrophage programming.
    Authors: Bi Y, Zhou J, Yang H, Wang X, Zhang X, Wang Q, Wu X, Han Y, Song Y, Tan Y, Du Z, Yang H, Zhou D, Cui Y, Zhou L, Yan Y, Zhang P, Guo Z, Wang X, Liu G, Yang R
    J Immunol, 2013-12-13;192(2):704-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta.
    Authors: Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S
    Int J Cancer, 2013-10-31;134(6):1346-58.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  28. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
    Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
    J Immunol, 2013-04-15;190(10):5329-36.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  29. Inefficient antimicrobial functions of innate phagocytes render infant mice more susceptible to bacterial infection.
    Authors: Zhang Q, Coveney A, Yu S, Liu J, Li Y, Blankson S, Redmond H, Wang J, Wang J
    Eur J Immunol, 2013-03-22;43(5):1322-32.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis.
    Authors: Roy M, Richard JF, Dumas A, Vallieres L
    J Neuroinflammation, 2012-01-23;9(0):18.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  31. TNF-alpha promotes LPA1- and LPA3-mediated recruitment of leukocytes in vivo through CXCR2 ligand chemokines.
    Authors: Zhao C, Sardella A, Chun J, Poubelle PE, Fernandes MJ, Bourgoin SG
    J. Lipid Res., 2011-04-26;52(7):1307-18.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  32. Defective regulation of CXCR2 facilitates neutrophil release from bone marrow causing spontaneous inflammation in severely NF-kappa B-deficient mice.
    Authors: von Vietinghoff S, Asagiri M, Azar D
    J. Immunol., 2010-06-02;185(1):670-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  33. Respiratory syncytial virus induces airway insensitivity to beta-agonists in BALB/c mice.
    Authors: Traylor ZP, Yu EN, Davis IC
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009-12-04;298(3):L437-45.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  34. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
    Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
    Proc. Natl. Acad. Sci. U.S.A., 2009-03-16;106(14):5954-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  35. Neuroprotection and remyelination after autoimmune demyelination in mice that inducibly overexpress CXCL1.
    Authors: Omari KM, Lutz SE, Santambrogio L, Lira SA, Raine CS
    Am. J. Pathol., 2008-12-18;174(1):164-76.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  36. Chemokine-mediated rapid turnover of myeloid-derived suppressor cells in tumor-bearing mice.
    Authors: Sawanobori Y, Ueha S, Kurachi M, Shimaoka T, Talmadge JE, Abe J, Shono Y, Kitabatake M, Kakimi K, Mukaida N, Matsushima K
    Blood, 2008-03-28;111(12):5457-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  37. An age-associated increase in pulmonary inflammation after burn injury is abrogated by CXCR2 inhibition.
    Authors: Nomellini V, Faunce DE, Gomez CR, Kovacs EJ
    J. Leukoc. Biol., 2008-03-13;83(6):1493-501.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  38. HIF-1alpha regulates epithelial inflammation by cell autonomous NFkappaB activation and paracrine stromal remodeling.
    Authors: Scortegagna M, Cataisson C, Martin RJ, Hicklin DJ, Schreiber RD, Yuspa SH, Arbeit JM
    Blood, 2008-01-16;111(7):3343-54.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  39. Anti-type II collagen antibody accelerates arthritis via CXCR2-expressing cells in IL-1 receptor antagonist-deficient mice.
    Authors: Kagari T, Tanaka D, Doi H, Iwakura Y, Shimozato T
    Eur. J. Immunol., 2007-10-01;37(10):2753-63.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Neutralization
  40. Genetic removal of the A2A adenosine receptor enhances pulmonary inflammation, mucin production, and angiogenesis in adenosine deaminase-deficient mice.
    Authors: Mohsenin A, Mi T, Xia Y, Kellems RE, Chen JF, Blackburn MR
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007-06-29;293(3):L753-61.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  41. An essential role for IL-17 in preventing pathogen-initiated bone destruction: recruitment of neutrophils to inflamed bone requires IL-17 receptor-dependent signals.
    Authors: Yu JJ, Ruddy MJ, Wong GC, Sfintescu C, Baker PJ, Smith JB, Evans RT, Gaffen SL
    Blood, 2007-01-03;109(9):3794-802.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  42. The Lineage-Defining Transcription Factors SOX2 and NkX2-1 Determine Lung Cancer Cell Fate and Shape the Tumor Immune Microenvironment.
    Authors: Mollaoglu G, Jones A, Wait SJ et al.
    Immunity.
  43. Cytotoxic T cells swarm by homotypic chemokine signalling
    Authors: Jorge Luis Galeano Niño, Sophie V Pageon, Szun S Tay, Feyza Colakoglu, Daryan Kempe, Jack Hywood et al.
    eLife
  44. Mesenchymal stem cells promote mammary cancer cell migRation in vitro via the CXCR2 receptor.
    Authors: Halpern Jennifer L, Kilbarger Amy, Lynch Conor C.
    Cancer Letters

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Mouse CXCR2/IL-8RB Antibody
By Anonymous on 08/18/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: fibroblasts Species: Mouse